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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- ELISA [1]
- Immunocytochemistry [1]
- Chromatin Immunoprecipitation [1]
- Other assay [1]
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- Product number
- GTX60828 - Provider product page
- Provider
- GeneTex
- Product name
- Histone H4K20me3 (trimethyl Lys20) antibody - ChIP grade
- Antibody type
- Polyclonal
- Reactivity
- Human, Mouse
- Host
- Rabbit
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Supportive validation
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- GeneTex (provider)
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- Experimental details
- WB analysis of whole cell (25 ?g, lane 1) and histone extracts (15 ?g, lane 2) from HeLa cells, and 1 ?g of recombinant histone H2A, H2B, H3 and H4 (lane 3, 4, 5 and 6, respectively) using H4K20me3 antibody at a dilution of 1:1,000 in TBS-Tween containing 5% skimmed milk.
- Validation comment
- WB
Supportive validation
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- GeneTex (provider)
- Main image
- Experimental details
- ELISA was performed using a serial dilution of Histone H4K20me3 (tri-Methyl Lys20) antibody in antigen coated wells. By plotting the absorbance against the antibody dilution, the titer of the antibody was estimated to be 1:21,700.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- ICC/IF analysis of HeLa cells using H4K20me3 antibody (green) and DAPI (blue). Cells were fixed with methanol and blocked with PBS/TX-100 containing 5% normal goat serum and 1% BSA. The cells were immunofluorescently labeled with H4K20me3 antibody at a dilution of 1:500.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- ChIP was performed with K562 extracts and either control IgG (1 μg) or Histone H4K20me3 (tri-Methyl Lys20) antibody (0.2, 0.5, 1 and 2 μg per ChIP experiment). The precipitated DNA was detected by qPCR with primers targeting to positive controls ZNF510 and the Sat2 satellite repeat region, and to negative controls the promoters of the active genes EIF4A2 and GAPDH.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Dot blot analysis of peptides containing histone modifications and the unmodified H4K20 using H4K20me3 antibody at a dilution of 1:20,000. One hundred to 0.2 pmol of the respective peptides were spotted on a membrane for analysis.
- Validation comment
- Dot
