Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [4]
- Immunocytochemistry [2]
- Immunoprecipitation [2]
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Validation data
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- Product number
- LS-C674406 - Provider product page
- Provider
- LSBio
- Product name
- HIST1H4I Antibody LS-C674406
- Antibody type
- Polyclonal
- Description
- Protein G purified
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Storage
- Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
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Enhanced validation
- Submitted by
- LSBio (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- Positive Western Blot detected in Hela whole cell lysate, 293 whole cell lysate, Jurkat whole cell lysate, HepG2 whole cell lysate(treated by 30mM sodium butyrate for 4h). All lanes: HIST1H4A antibody at 2.2 µg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 12 KDa. Observed band size: 12 KDa
- Submitted by
- LSBio (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- Western Blot Detected samples: Hela whole cell lysate, Jurkat whole cell lysate, 293 whole cell lysate; Untreated (-) or treated (+) with 10mM sodium propionate for 4h All lanes: HIST1H4A antibody at 1:1000 Secondary Goat polyclonal to rabbit IgG at 1/40000 dilution Predicted band size: 12 kDa Observed band size: 12 kDa
- Submitted by
- LSBio (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- Western Blot Detected samples: Hela whole cell lysate, Jurkat whole cell lysate, 293 whole cell lysate; Untreated (-) or treated (+) with 10mM sodium propionate for 4h All lanes: HIST1H4A antibody at 1:1000 Secondary Goat polyclonal to rabbit IgG at 1/40000 dilution Predicted band size: 12 kDa Observed band size: 12 kDa
- Submitted by
- LSBio (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- Positive Western Blot detected in Hela whole cell lysate, 293 whole cell lysate, Jurkat whole cell lysate, HepG2 whole cell lysate(treated by 30mM sodium butyrate for 4h). All lanes: HIST1H4A antibody at 2.2 µg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 12 KDa. Observed band size: 12 KDa
Supportive validation
- Submitted by
- LSBio (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- Immunocytochemistry analysis of Propionyl-HIST1H4A (K5) Antibody diluted at 1:100 and staining in Hela cells (treated with 10mM sodium propionate for 4h) performed on a Leica BondTM system. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
- Submitted by
- LSBio (provider)
- Main image
- Experimental details
- Immunocytochemistry analysis of Propionyl-HIST1H4A (K5) Antibody diluted at 1:100 and staining in Hela cells (treated with 10mM sodium propionate for 4h) performed on a Leica BondTM system. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
Supportive validation
- Submitted by
- LSBio (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- Immunoprecipitating HIST1H4A in Hela whole cell lysate Lane 1: Rabbit control IgG instead of Propionyl-HIST1H4A (K5) Antibody in Hela whole cell lysate.For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000) Lane 2: Propionyl-HIST1H4A (K5) Antibody (5µg) + Hela whole cell lysate (500µg) Lane 3: Hela whole cell lysate (20µg)
- Submitted by
- LSBio (provider)
- Main image
- Experimental details
- Immunoprecipitating HIST1H4A in Hela whole cell lysate Lane 1: Rabbit control IgG instead of Propionyl-HIST1H4A (K5) Antibody in Hela whole cell lysate.For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000) Lane 2: Propionyl-HIST1H4A (K5) Antibody (5µg) + Hela whole cell lysate (500µg) Lane 3: Hela whole cell lysate (20µg)