Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- Immunoprecipitation [2]
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Validation data
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- Product number
- LS-C676965 - Provider product page
- Provider
- LSBio
- Product name
- HIST1H4I Antibody LS-C676965
- Antibody type
- Polyclonal
- Description
- Immunoaffinity purified
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Storage
- Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
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Enhanced validation
- Submitted by
- LSBio (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- Western Blot Positive WB detected in: Hela whole cell lysate, 293 whole cell lysate, A549 whole cell lysate, K562 whole cell lysate, HepG2 whole cell lysate, Mouse kidney tissue All lanes: HIST1H4A antibody at 3.2µg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 12 kDa Observed band size: 12 kDa
- Submitted by
- LSBio (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- Western Blot Positive WB detected in: Hela whole cell lysate, 293 whole cell lysate, A549 whole cell lysate, K562 whole cell lysate, HepG2 whole cell lysate, Mouse kidney tissue All lanes: HIST1H4A antibody at 3.2µg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 12 kDa Observed band size: 12 kDa
Supportive validation
- Submitted by
- LSBio (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- Immunoprecipitating HIST1H4A in Hela whole cell lysate Lane 1: Rabbit control IgG instead of HIST1H4A (Ab-12) Antibody in Hela whole cell lysate.For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000) Lane 2: HIST1H4A (Ab-12) Antibody (8µg) + Hela whole cell lysate (500µg) Lane 3: Hela whole cell lysate (20µg)
- Submitted by
- LSBio (provider)
- Main image
- Experimental details
- Immunoprecipitating HIST1H4A in Hela whole cell lysate Lane 1: Rabbit control IgG instead of HIST1H4A (Ab-12) Antibody in Hela whole cell lysate.For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000) Lane 2: HIST1H4A (Ab-12) Antibody (8µg) + Hela whole cell lysate (500µg) Lane 3: Hela whole cell lysate (20µg)