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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [2]
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- Validations
- Western blot [2]
- Immunoprecipitation [1]
- Immunohistochemistry [2]
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- Product number
- NB100-2129 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NB100-2129, RRID:AB_2281537
- Product name
- Rabbit Polyclonal MAML3 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified.
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 0.1 ml
- Concentration
- 0.2 mg/ml
- Storage
- Store at 4C. Do not freeze.
Submitted references Optical physics: How to hide in time.
High-level expression of Mastermind-like 2 contributes to aberrant activation of the NOTCH signaling pathway in human lymphomas.
Boyd RW, Shi Z
Nature 2012 Jan 4;481(7379):35-6
Nature 2012 Jan 4;481(7379):35-6
High-level expression of Mastermind-like 2 contributes to aberrant activation of the NOTCH signaling pathway in human lymphomas.
Köchert K, Ullrich K, Kreher S, Aster JC, Kitagawa M, Jöhrens K, Anagnostopoulos I, Jundt F, Lamprecht B, Zimber-Strobl U, Stein H, Janz M, Dörken B, Mathas S
Oncogene 2011 Apr 14;30(15):1831-40
Oncogene 2011 Apr 14;30(15):1831-40
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: MAML3 Antibody [NB100-2129] - Western Blot and Immunoprecipitation. Whole cell lysate from HeLa, 293T and mouse NIH3T3 cells. Affinity purified rabbit anti-MAML3 antibody NB100-2129. MAML3 was also immunoprecipitated by rabbit anti-MAML3 NB100-2128.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: MAML3 Antibody [NB100-2129] - Detection of Human and Mouse MAML3 by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa and mouse NIH3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-MAML3 antibody NB100-2129 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunoprecipitation: MAML3 Antibody [NB100-2129] - Detection of human MAML3 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HEK293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-MAML3 antibody NB100-2129 (lot NB100-2129-2) used for IP at 6 ug per reaction. MAML3 was also immunoprecipitated by a previous lot of this antibody (lot NB100-2129-1) and rabbit anti-MAML3 antibody NB100-2128 For blotting immunoprecipitated MAML3, NB100-2129 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry: MAML3 Antibody [NB100-2129] - Sample: FFPE section of human breast carcinoma and mouse teratoma. Antibody: Affinity purified rabbit anti-MAML3 used at a dilution of 1:200 (1ug/ml). Detection: DAB
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: MAML3 Antibody [NB100-2129] - Detection of human MAML3 by immunohistochemistry. Sample: FFPE section of human prostate carcinoma. Antibody: Affinity purified rabbit anti-MAML3 (NB100-2129). Detection: DAB
