16660-1-AP

antibody from Invitrogen Antibodies

Targeting: DDX52 ROK1

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

16660-1-AP

Provider

Invitrogen Antibodies

Product name

DDX52 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Other

Description

Immunogen sequence: TRELASQIH RELIKISEGT GFRIHMIHKA AVAAKKFGPK SSKKFDILVT TPNRLIYLLK QDPPGIDLAS VEWLVVDESD KLFEDGKTGF RDQLASIFLA CTSHKVRRAM FSATFAYDVE QWCKLNLDNV ISVSIGARNS AVETVEQELL FVGSETGKLL AVRELVKKGF NPPVLVFVQS IERAKELFHE LIYEGINVDV IHAERTQQQR DNTVHSFRAG KIWVLICTAL LARGIDFKGV NLVINYDFPT SSVEYIHRIG RTGRAGNKGK AITFFTEDDK PLLRSVANVI QQAGCPVPEY IKGFQKLLSK QKKKMIKKPL ERESISTTPK CFLEKAKDKQ KKVTGQNSKK KVALEDKS (243-599 aa encoded by BC041785)

Reactivity

Human, Mouse, Rat

Host

Rabbit

Isotype

IgG

Vial size

150 µL

Concentration

0.13 mg/mL

Storage

-20°C

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Various lysates were subjected to SDS PAGE followed by western blot with 16660-1-AP (DDX52 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Jurkat cells were subjected to SDS PAGE followed by western blot with 16660-1-AP (DDX52 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Various lysates were subjected to SDS PAGE followed by western blot with 16660-1-AP (DDX52 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

HepG2 cells were subjected to SDS PAGE followed by western blot with 16660-1-AP (DDX52 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of ( 10% Formaldehyde ) fixed HepG2 cells using 16660-1-AP (DDX52 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of ( 10% Formaldehyde ) fixed HepG2 cells using 16660-1-AP (DDX52 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L).