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- Antibody Data
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- Immunohistochemistry [2]
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- Product number
- AF3586 - Provider product page
- Provider
- R&D Systems
- Product name
- Human Trypsin Pan Specific (PRSS1/2/3) Antibody
- Antibody type
- Polyclonal
- Description
- Antigen Affinity-purified. Detects human Trypsin Pan Specific (PRSS1/2/3) in direct ELISAs and Western blots.
- Reactivity
- Human
- Host
- Sheep
- Conjugate
- Unconjugated
- Antigen sequence
P07478- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
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Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Trypsin in Human Pancreas. Trypsin was detected in immersion fixed paraffin-embedded sections of human pancreas using Sheep Anti-Human Trypsin Pan Specific (PRSS1/2/3) Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3586) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Sheep HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS019) and counterstained with hematoxylin (blue). Specific staining was localized to exocrine cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Trypsin in Human Pancreatic Cancer Tissue. Trypsin was detected in immersion fixed paraffin-embedded sections of human pancreatic cancer tissue using Sheep Anti-Human Trypsin Pan Specific (PRSS1/2/3) Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3586) at 5 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Sheep HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS019) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm of cancer cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
