MA5-34694

antibody from Invitrogen Antibodies

Targeting: NPC1 SLC65A1

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

MA5-34694

Provider

Invitrogen Antibodies

Product name

NPC1 Recombinant Rabbit Monoclonal Antibody (JB87-33)

Antibody type

Monoclonal

Antigen

Recombinant full-length protein

Description

Positive Control: SiHa, A549, HepG2, PC-3M, Rat kidney tissue, human kidney tissue, mouse testis tissue, SH-SY-5Y.

Reactivity

Human

Host

Rabbit

Isotype

IgG

Antibody clone number

JB87-33

Vial size

100 µL

Concentration

1 mg/mL

Storage

-20° C, Avoid Freeze/Thaw Cycles, store in dark

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of Niemann Pick C1 in SiHa cell lysate. Samples were incubated with Niemann Pick C1 monoclonal antibody (Product # MA5-34694), at a dilution of 1:500.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of Niemann Pick C1 in PC-3M cells (green). Samples were fixed in paraformaldehyde and permeabilised with 0.25% Triton X100/PBS, incubated with Niemann Pick C1 monoclonal antibody (Product # MA5-34694), followed by DAPI (blue).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of Niemann Pick C1 in HepG2 cells (green). Samples were fixed in paraformaldehyde and permeabilised with 0.25% Triton X100/PBS, incubated with Niemann Pick C1 monoclonal antibody (Product # MA5-34694), followed by DAPI (blue).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of Niemann Pick C1 in A549 cells (green). Samples were fixed in paraformaldehyde and permeabilised with 0.25% Triton X100/PBS, incubated with Niemann Pick C1 monoclonal antibody (Product # MA5-34694), followed by DAPI (blue).

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis of Niemann Pick C1 in paraffin-embedded rat kidney tissue. Samples were incubated with Niemann Pick C1 monoclonal antibody (Product # MA5-34694), and followed by hematoxylin.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis of Niemann Pick C1 in paraffin-embedded mouse testis tissue. Samples were incubated with Niemann Pick C1 monoclonal antibody (Product # MA5-34694), and followed by hematoxylin.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis of Niemann Pick C1 in paraffin-embedded human kidney tissue. Samples were incubated with Niemann Pick C1 monoclonal antibody (Product # MA5-34694), and followed by hematoxylin.

Flow cytometry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Flow cytometry of Niemann Pick C1 in SH-SY-5Y cells (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Samples were incubated with Niemann Pick C1 monoclonal antibody (Product # MA5-34694) at a dilution of 1:100, followed by Alexa Fluor 488-conjugated goat anti rabbit IgG.