Antibody data
- Antibody Data
- Antigen structure
- References [5]
- Comments [0]
- Validations
- Western blot [1]
- ELISA [1]
- Immunohistochemistry [1]
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- Product number
- ABIN393698 - Provider product page
- Provider
- antibodies-online
- Product name
- anti-Ring Finger Protein 139 (RNF139) (AA 565-665) antibody
- Antibody type
- Monoclonal
- Description
- This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
- Reactivity
- Human
- Host
- Mouse
- Epitope
- AA 565-665
- Isotype
- IgG
- Antibody clone number
- 3D10
- Vial size
- 100 μg
- Storage
- Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
Submitted references The TRC8 ubiquitin ligase is sterol regulated and interacts with lipid and protein biosynthetic pathways.
The TRC8 E3 ligase ubiquitinates MHC class I molecules before dislocation from the ER.
The sterol-sensing endoplasmic reticulum (ER) membrane protein TRC8 hampers ER to Golgi transport of sterol regulatory element-binding protein-2 (SREBP-2)/SREBP cleavage-activated protein and reduces SREBP-2 cleavage.
A constitutional balanced t(3;8)(p14;q24.1) translocation results in disruption of the TRC8 gene and predisposition to clear cell renal cell carcinoma.
Global, in vivo, and site-specific phosphorylation dynamics in signaling networks.
Lee JP, Brauweiler A, Rudolph M, Hooper JE, Drabkin HA, Gemmill RM
Molecular cancer research : MCR 2010 Jan;8(1):93-106
Molecular cancer research : MCR 2010 Jan;8(1):93-106
The TRC8 E3 ligase ubiquitinates MHC class I molecules before dislocation from the ER.
Stagg HR, Thomas M, van den Boomen D, Wiertz EJ, Drabkin HA, Gemmill RM, Lehner PJ
The Journal of cell biology 2009 Sep 7;186(5):685-92
The Journal of cell biology 2009 Sep 7;186(5):685-92
The sterol-sensing endoplasmic reticulum (ER) membrane protein TRC8 hampers ER to Golgi transport of sterol regulatory element-binding protein-2 (SREBP-2)/SREBP cleavage-activated protein and reduces SREBP-2 cleavage.
Irisawa M, Inoue J, Ozawa N, Mori K, Sato R
The Journal of biological chemistry 2009 Oct 16;284(42):28995-9004
The Journal of biological chemistry 2009 Oct 16;284(42):28995-9004
A constitutional balanced t(3;8)(p14;q24.1) translocation results in disruption of the TRC8 gene and predisposition to clear cell renal cell carcinoma.
Poland KS, Azim M, Folsom M, Goldfarb R, Naeem R, Korch C, Drabkin HA, Gemmill RM, Plon SE
Genes, chromosomes & cancer 2007 Sep;46(9):805-12
Genes, chromosomes & cancer 2007 Sep;46(9):805-12
Global, in vivo, and site-specific phosphorylation dynamics in signaling networks.
Olsen JV, Blagoev B, Gnad F, Macek B, Kumar C, Mortensen P, Mann M
Cell 2006 Nov 3;127(3):635-48
Cell 2006 Nov 3;127(3):635-48
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- ELISA
Supportive validation
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- Experimental details
- IHC