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Western blot
ELISAAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
- Immunohistochemistry [1]
- Flow cytometry [1]
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- Product number
- AP50171PU-N - Provider product page
- Provider
- Acris Antibodies GmbH
- Proper citation
- Acris Antibodies GmbH Cat#AP50171PU-N, RRID:AB_11146718
- Product name
- anti APC10 / ANAPC10 (N-term)
- Antibody type
- Polyclonal
- Antigen
- KLH conjugated synthetic peptide between 3~32 amino acids from the N-terminal region of human ANAPC10
- Reactivity
- Human
- Host
- Rabbit
- Vial size
- 0.1 mg
- Concentration
- 0.25 mg/ml
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Supportive validation
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- Western blot analysis of ANAPC10 Antibody (N-term) (Cat. #AP50171PU-N) in WiDr cell line lysates (35µg/lane). ANAPC10 (arrow) was detected using the purified Pab.
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- Western blot analysis of ANAPC10 (arrow) using rabbit polyclonal ANAPC10 Antibody (N-term) (Cat. #AP50171PU-N). 293 cell lysates (2 µg/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the ANAPC10 gene.
Supportive validation
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- Formalin-fixed and paraffin-embedded human brain tissue reacted with ANAPC10 Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Supportive validation
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- ANAPC10 Antibody (N-term) (Cat. #AP50171PU-N) flow cytometric analysis of WiDr cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
