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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [3]
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- Validations
- Western blot [2]
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- Product number
- PAB10019 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#PAB10019, RRID:AB_1671769
- Product name
- ANAPC10 polyclonal antibody
- Antibody type
- Polyclonal
- Description
- Rabbit polyclonal antibody raised against synthetic peptide of ANAPC10.
- Storage
- Store at 4°C. For long term storage store at -20°C.Aliquot to avoid repeated freezing and thawing.
Submitted references Doc1 mediates the activity of the anaphase-promoting complex by contributing to substrate recognition.
Activity of the APC(Cdh1) form of the anaphase-promoting complex persists until S phase and prevents the premature expression of Cdc20p.
Characterization of the DOC1/APC10 subunit of the yeast and the human anaphase-promoting complex.
Passmore LA, McCormack EA, Au SW, Paul A, Willison KR, Harper JW, Barford D
The EMBO journal 2003 Feb 17;22(4):786-96
The EMBO journal 2003 Feb 17;22(4):786-96
Activity of the APC(Cdh1) form of the anaphase-promoting complex persists until S phase and prevents the premature expression of Cdc20p.
Huang JN, Park I, Ellingson E, Littlepage LE, Pellman D
The Journal of cell biology 2001 Jul 9;154(1):85-94
The Journal of cell biology 2001 Jul 9;154(1):85-94
Characterization of the DOC1/APC10 subunit of the yeast and the human anaphase-promoting complex.
Grossberger R, Gieffers C, Zachariae W, Podtelejnikov AV, Schleiffer A, Nasmyth K, Mann M, Peters JM
The Journal of biological chemistry 1999 May 14;274(20):14500-7
The Journal of biological chemistry 1999 May 14;274(20):14500-7
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Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Immunoblotting of ANAPC10 polyclonal antibody (Cat # PAB10019) was used at a 1:500 dilution to detect human ANAPC10 by western blot.Both HeLa whole cell lysate (Lane 1) and nuclear lysate (Lane 2) were probed using this antibody.Approximately 20 ug of each lysate was loaded onto a 10% SDS-PAGE gel.Primary antibody was reacted with the membrane at room temperature for 1 h.After subsequent washing, a 1 : 2,000 dilution of HRP conjugated Gt-a-Rabbit IgG was used for visualization.Exposure time was 5 min.The expected molecular weight of human ANAPC10 is 21 KDa.
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Immunoblotting of ANAPC10 polyclonal antibody (Cat # PAB10019) was used at a 1:500 dilution to detect human ANAPC10 in various cell extracts.This antibody clearly detects a ~26 KDa band corresponding to human ANAPC10 (predicted molecular weight is 21 KDa).All lanes contain 20 ug of lysate or extract as follows : lane 1, HeLa nuclear extract; lane 2, HeLa whole cell lysate; lane 3, A-431 whole cell lysate; lane 4, Jurkat whole cell lysate; lane 5, 293 whole cell lysate.Primary antibody was reacted with the membrane at room temperature for 1 h.After subsequent washing, a 1 : 5,000 dilution of HRP conjugated Gt-a-Rabbit IgG was used for visualization.Exposure time was 4 min.
