Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
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Validation data
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- Product number
- AF7618 - Provider product page
- Provider
- R&D Systems
- Product name
- Human Glis1 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified. Detects human Glis1 in direct ELISAs and Western blots. In direct ELISAs, approximately 60% cross-reactivity with recombinant mouse Glis1 is observed.
- Reactivity
- Human
- Host
- Sheep
- Conjugate
- Unconjugated
- Antigen sequence
Q5VTL4
- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
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Supportive validation
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- R&D Systems (provider)
- Main image
- Experimental details
- Detection of Human Glis1 by Western Blot. Western blot shows lysates of HEK001 human epidermal keratinocyte cell line, WS-1 human fetal skin fibroblast cell line, U-118-MG human glioblastoma/astrocytoma cell line, and U-87 MG human glioblastoma/astrocytoma cell line. PVDF membrane was probed with 2 µg/mL of Sheep Anti-Human Glis1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7618) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Glis1 at approximately 95 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.