STJ96979

antibody from St John's Laboratory

Targeting: CASP9 APAF-3, ICE-LAP6, MCH6, PPP1R56

Immunocytochemistry

Immunoprecipitation

Antibody data

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Validation data

Product number: STJ96979 - Provider product page
Provider: St John's Laboratory
Product name: Anti-CASP9 antibody [3-20] (STJ96979)
Antibody type: Monoclonal
Description: Mouse monoclonal antibody anti-Caspase-9 is suitable for use in Western Blot, Immunohistochemistry, Immunofluorescence, Immunocytochemistry and Immunoprecipitation research applications.
Reactivity: Human, Mouse, Rat, Chicken/Avian
Host: Mouse
Conjugate: Unconjugated
Antigen sequence: NA
Epitope: NA
Isotype: IgG
Antibody clone number: NA
Vial size: NA
Concentration: NA
Storage: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Handling: NA

CASP9 protein structure - STJ96979 shown in red.

Supportive validation

Submitted by: St John's Laboratory (provider)
Main image
Experimental details: Western blot analysis of chicken cell lysis using Antibody diluted at 1:1000
Sample type: NA
Validation comment: NA
Primary Ab dilution: NA
Other comments: NA
Secondary Ab: NA
Secondary Ab dilution: NA
Protocol: NA
Appendix: NA

Supportive validation

Submitted by: St John's Laboratory (provider)
Main image
Experimental details: Western blot analysis of lysates from 1) Hela, 2) Jurkat, 3) 3T3 cells, (Green) primary antibody was diluted at 1:1000, 4Â°C over night, secondary antibody (cat: (NA) was diluted at 1:10000, 37Â°C 1hour. (Red) GAPDH Polyclonal Antibody (cat: (STJ97090) antibody was diluted at 1:5000 as loading control, 4Â°C over night, secondary antibody (cat: (NA) was diluted at 1:10000, 37Â°C 1hour.
Sample type: NA
Validation comment: NA
Primary Ab dilution: NA
Other comments: NA
Secondary Ab: NA
Secondary Ab dilution: NA
Protocol: NA
Appendix: NA

Supportive validation

Submitted by: St John's Laboratory (provider)
Main image
Experimental details: Western blot analysis of Hela, diluted at, 1) 1:2000 , 2) 1:5000
Sample type: NA
Validation comment: NA
Primary Ab dilution: NA
Other comments: NA
Secondary Ab: NA
Secondary Ab dilution: NA
Protocol: NA
Appendix: NA

Supportive validation

Submitted by: St John's Laboratory (provider)
Main image
Experimental details: Immunofluorescence analysis of Mouse-brain tissue. 1, Caspase 9 monoclonal antibody (3-20) (red) was diluted at 1:200 (4°C, overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300 (room temperature, 50min).3, Picture B: DAPI (blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B
Sample type: NA
Validation comment: NA
Primary Ab dilution: NA
Other comments: NA
Secondary Ab: NA
Secondary Ab dilution: NA
Protocol: NA
Appendix: NA

Supportive validation

Submitted by: St John's Laboratory (provider)
Main image
Experimental details: Immunofluorescence analysis of Rat-spleen tissue. 1, Caspase 9 monoclonal antibody (3-20) (red) was diluted at 1:200 (4°C, overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300 (room temperature, 50min).3, Picture B: DAPI (blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B
Sample type: NA
Validation comment: NA
Primary Ab dilution: NA
Other comments: NA
Secondary Ab: NA
Secondary Ab dilution: NA
Protocol: NA
Appendix: NA

Supportive validation

Submitted by: St John's Laboratory (provider)
Main image
Experimental details: Immunofluorescence analysis of Human-appendix tissue. 1, Caspase 9 monoclonal antibody (3-20) (red) was diluted at 1:200 (4°C, overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300 (room temperature, 50min).3, Picture B: DAPI (blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B
Sample type: NA
Validation comment: NA
Primary Ab dilution: NA
Other comments: NA
Secondary Ab: NA
Secondary Ab dilution: NA
Protocol: NA
Appendix: NA

Supportive validation

Submitted by: St John's Laboratory (provider)
Main image
Experimental details: Immunofluorescence analysis of Hela cell. 1, eIF2 Alpha Polyclonal Antibody (red) was diluted at 1:200 (4°C overnight). Caspase 9 monoclonal antibody (3-20) (green) was diluted at 1:200 (4°C overnight). 2, Goat Anti Rabbit Alexa Fluor 594 Catalog: (NA was diluted at 1:1000 (room temperature, 50min). Goat Anti Mouse Alexa Fluor 488 Catalog: (NA was diluted at 1:1000 (room temperature, 50min).
Sample type: NA
Validation comment: NA
Primary Ab dilution: NA
Other comments: NA
Secondary Ab: NA
Secondary Ab dilution: NA
Protocol: NA
Appendix: NA

Supportive validation

Submitted by: St John's Laboratory (provider)
Main image
Experimental details: Immunohistochemical analysis of paraffin-embedded Mouse-kidney tissue. 1, Caspase 9 monoclonal antibody (3-20) was diluted at 1:200 (4Â°C, overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval (>98Â°C, 20min). 3, Secondary antibody was diluted at 1:200 (room tempeRature, 30min). Negative control was used by secondary antibody only.
Sample type: NA
Validation comment: NA
Primary Ab dilution: NA
Other comments: NA
Secondary Ab: NA
Secondary Ab dilution: NA
Protocol: NA
Appendix: NA

Supportive validation

Submitted by: St John's Laboratory (provider)
Main image
Experimental details: Immunohistochemical analysis of paraffin-embedded Rat-lung tissue. 1, Caspase 9 monoclonal antibody (3-20) was diluted at 1:200 (4Â°C, overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval (>98Â°C, 20min). 3, Secondary antibody was diluted at 1:200 (room tempeRature, 30min). Negative control was used by secondary antibody only.
Sample type: NA
Validation comment: NA
Primary Ab dilution: NA
Other comments: NA
Secondary Ab: NA
Secondary Ab dilution: NA
Protocol: NA
Appendix: NA

Supportive validation

Submitted by: St John's Laboratory (provider)
Main image
Experimental details: Immunohistochemical analysis of paraffin-embedded Human-lung tissue. 1, Caspase 9 monoclonal antibody (3-20) was diluted at 1:200 (4Â°C, overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval (>98Â°C, 20min). 3, Secondary antibody was diluted at 1:200 (room tempeRature, 30min). Negative control was used by secondary antibody only.
Sample type: NA
Validation comment: NA
Primary Ab dilution: NA
Other comments: NA
Secondary Ab: NA
Secondary Ab dilution: NA
Protocol: NA
Appendix: NA