621901

antibody from BioLegend
Targeting: CASP9 APAF-3, ICE-LAP6, MCH6, PPP1R56
Provider product page for 621901
Western blot
Immunoprecipitation

Antibody data

Product number
621901 - Provider product page
Provider
BioLegend
Proper citation
BioLegend Cat#621901, RRID:AB_315941
Product name
Purified anti-Caspase-9
Antibody type
Monoclonal
Antigen
N-terminal fragment of human caspase-9
Description
Caspase 9 (also known as ICE-like apoptotic protease 6 (ICE-LAP6), apoptotic protease Mch-6, and apoptotic protease activating factor 3 (Apaf-3)) is a member of the peptidase family C14 that contains a CARD domain. This caspase is active as a heterotetramer and has been reported to have two isoforms. Pro-Caspase 9 has been reported to be approximately 47 kD. This caspase is present in the cytosol and, upon activation, translocates to the mitochondria. Caspase 9 is involved in the caspase activation cascade responsible for apoptosis execution and cleaves/activates Caspase 3 and Caspase 6. Caspase 9 is inhibited by the dominant negative isoform, Bcl-XL, c-IAP1, c-IAP2, XIAP, and Livin. This caspase becomes activated when recruited to Apaf-1/cytochrome c complex, and following cleavage by Apaf-1, granzyme B, Caspase 3, possibly Caspase 8 and Caspase 10 into large p37 and small p10 subunits. Caspase 9 interacts with BIRC7 and has been shown to cleave PARP and vimentin. The 96-2-22 monoclonal antibody has been shown to be useful for Western blotting of human caspase 9 (46 kD pro-caspase 9 as well as the 34 kD cleaved caspase 9).
Reactivity
Human
Host
Mouse
Conjugate
Unconjugated
Isotype
IgG
Vial size
25 µg
Concentration
0.5 mg/ml
Storage
Upon receipt, store at 4° C.
CASP9 protein structure - 621901 shown in red.