Antibody data
- Antibody Data
- Antigen structure
- References [11]
- Comments [0]
- Validations
- Western blot [4]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
- Other assay [2]
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Validation data
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- Product number
- PA5-16358 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Caspase 9 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- The recommended shelf life for this product is 1 year from date of receipt.
- Reactivity
- Human, Mouse, Rat, Bovine
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 500 µL
- Concentration
- 1 mg/mL
- Storage
- 4° C
Submitted references LDHB inhibition induces mitophagy and facilitates the progression of CSFV infection.
Antibody-drug nanoparticle induces synergistic treatment efficacies in HER2 positive breast cancer cells.
Parthenolide inhibits the proliferation and induces the apoptosis of human uveal melanoma cells.
Geraniol attenuates 2-acetylaminofluorene induced oxidative stress, inflammation and apoptosis in the liver of wistar rats.
miR-181b as a therapeutic agent for chronic lymphocytic leukemia in the Eµ-TCL1 mouse model.
Glycyrrhizic acid suppresses the development of precancerous lesions via regulating the hyperproliferation, inflammation, angiogenesis and apoptosis in the colon of Wistar rats.
Hsp90-targeted miRNA-liposomal formulation for systemic antitumor effect.
Treatment of vitiligo with a chimeric monoclonal antibody to CD20: a pilot study.
Expression of Bak and Bak/Mcl-1 ratio can predict photodynamic therapy outcome for oral verrucous hyperplasia and leukoplakia.
Effect of valproic acid treatment on penile structure in prepubertal rats.
N-acetylcysteine may prevent severe intestinal damage in necrotizing enterocolitis.
Fan S, Wu K, Zhao M, Yuan J, Ma S, Zhu E, Chen Y, Ding H, Yi L, Chen J
Autophagy 2021 Sep;17(9):2305-2324
Autophagy 2021 Sep;17(9):2305-2324
Antibody-drug nanoparticle induces synergistic treatment efficacies in HER2 positive breast cancer cells.
Abedin MR, Powers K, Aiardo R, Barua D, Barua S
Scientific reports 2021 Apr 1;11(1):7347
Scientific reports 2021 Apr 1;11(1):7347
Parthenolide inhibits the proliferation and induces the apoptosis of human uveal melanoma cells.
Che ST, Bie L, Li X, Qi H, Yu P, Zuo L
International journal of ophthalmology 2019;12(10):1531-1538
International journal of ophthalmology 2019;12(10):1531-1538
Geraniol attenuates 2-acetylaminofluorene induced oxidative stress, inflammation and apoptosis in the liver of wistar rats.
Hasan SK, Sultana S
Toxicology mechanisms and methods 2015;25(7):559-73
Toxicology mechanisms and methods 2015;25(7):559-73
miR-181b as a therapeutic agent for chronic lymphocytic leukemia in the Eµ-TCL1 mouse model.
Bresin A, Callegari E, D'Abundo L, Cattani C, Bassi C, Zagatti B, Narducci MG, Caprini E, Pekarsky Y, Croce CM, Sabbioni S, Russo G, Negrini M
Oncotarget 2015 Aug 14;6(23):19807-18
Oncotarget 2015 Aug 14;6(23):19807-18
Glycyrrhizic acid suppresses the development of precancerous lesions via regulating the hyperproliferation, inflammation, angiogenesis and apoptosis in the colon of Wistar rats.
Khan R, Khan AQ, Lateef A, Rehman MU, Tahir M, Ali F, Hamiza OO, Sultana S
PloS one 2013;8(2):e56020
PloS one 2013;8(2):e56020
Hsp90-targeted miRNA-liposomal formulation for systemic antitumor effect.
Pore SK, Choudhary A, Rathore B, Ganguly A, Sujitha P, Kumar CG, Agawane SB, Kumar JM, Scaria V, Pillai B, Banerjee R
Biomaterials 2013 Sep;34(28):6804-17
Biomaterials 2013 Sep;34(28):6804-17
Treatment of vitiligo with a chimeric monoclonal antibody to CD20: a pilot study.
Ruiz-Argüelles A, García-Carrasco M, Jimenez-Brito G, Sánchez-Sosa S, Pérez-Romano B, Garcés-Eisele J, Camacho-Alarcón C, Reyes-Núñez V, Sandoval-Cruz M, Mendoza-Pinto C, López-Colombo A
Clinical and experimental immunology 2013 Nov;174(2):229-36
Clinical and experimental immunology 2013 Nov;174(2):229-36
Expression of Bak and Bak/Mcl-1 ratio can predict photodynamic therapy outcome for oral verrucous hyperplasia and leukoplakia.
Yu CH, Chen HM, Lin HP, Chiang CP
Journal of oral pathology & medicine : official publication of the International Association of Oral Pathologists and the American Academy of Oral Pathology 2013 Mar;42(3):257-62
Journal of oral pathology & medicine : official publication of the International Association of Oral Pathologists and the American Academy of Oral Pathology 2013 Mar;42(3):257-62
Effect of valproic acid treatment on penile structure in prepubertal rats.
Kutlu O, Cansu A, Karagüzel E, Gürgen SG, Koç O, Gür M, Ozgür GK
Epilepsy research 2012 May;99(3):306-11
Epilepsy research 2012 May;99(3):306-11
N-acetylcysteine may prevent severe intestinal damage in necrotizing enterocolitis.
Tayman C, Tonbul A, Kosus A, Hirfanoglu IM, Uysal S, Haltas H, Tatli MM, Andiran F
Journal of pediatric surgery 2012 Mar;47(3):540-50
Journal of pediatric surgery 2012 Mar;47(3):540-50
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot of Caspase 9 using Caspase 9 Polyclonal Antibody (Product # PA5-16358) on Jurkat Cells.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Knockdown of Caspase 9 was achieved by transfecting HeLa cells with Caspase 9 specific siRNAs (Silencer® select Product # s2429, s2430). Western blot analysis (Fig. a) was performed using membrane enriched extracts from the Caspase 9 knockdown cells (lane 3), non-specific scrambled siRNA transfected cells (lane 2) and untransfected cells (lane 1). The blots were probed with Anti-Caspase 9 Polyclonal Antibody (Product # PA5-16358, 1 µg/mL) and detected by chemiluminescence using Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate (Product # A27036, 0.25 µg/mL, 1:4000 dilution). Densitometric analysis of this western blot is shown in histogram (Fig. b). Decrease in signal upon siRNA mediated knock down confirms that antibody is specific to Caspase 9.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis was performed on whole cell extracts (30 µg lysate) of HeLa (Lane 1), MCF7 (Lane 2), Jurkat (Lane 3), K-562 (Lane 4), HT-29 (Lane 5) and PC-3 (Lane 6). The blot was probed with Anti-Caspase 9 Rabbit Polyclonal Antibody (Product # PA5-16358, 3 µg/mL) and detected by chemiluminescence using Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate (Product # A28177, 0.25 µg/mL, 1:4000 dilution). A 45 kDa band corresponding to Caspase 9 was observed across the cell lines tested. Known quantity of protein samples were electrophoresed using Novex® NuPAGE® 4-12 % Bis-Tris gel (Product # NP0321BOX), XCell SureLock™ Electrophoresis System (Product # EI0002) and Novex® Sharp Pre-Stained Protein Standard (Product # LC5800). Resolved proteins were then transferred onto a nitrocellulose membrane with iBlot® 2 Dry Blotting System (Product # IB21001). The membrane was probed with the relevant primary and secondary Antibody following blocking with 5 % skimmed milk. Chemiluminescent detection was performed using Pierce™ ECL Western Blotting Substrate (Product # 32106).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis was performed on whole cell extracts (30 µg lysate) of A-431 (Lane 1), U-87 MG (Lane 2), HeLa (Lane 3). HeLa treated with Staurosporine (1uM for 3 hours) (Lane 4) and Jurkat (Lane 5). The blot was probed with Anti-Caspase 9 Polyclonal Antibody (Product # PA5-16358, 1 µg/mL) and detected by chemiluminescence using Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate (Product # A27036, 0.25 µg/mL, 1:4000 dilution). A 46 kDa band corresponding to Caspase 9 was observed across the cell lines tested and bands at 39 & 37 kDa was also observed corresponding to cleaved Caspase 9 following Staurosporine treatment.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescence analysis of Caspase 9 was performed using 70% confluent log phase HeLa cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with Caspase 9 Rabbit Polyclonal Antibody (Product # PA5-16358) at 2 µg/mL in 0.1% BSA and incubated for 3 hours at room temperature and then labeled with Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A27034) at a dilution of 1:2000 for 45 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with SlowFade® Gold Antifade Mountant with DAPI (Product # S36938). F-actin (Panel c: red) was stained with Rhodamine Phalloidin (Product # R415, 1:300). Panel d represents the merged image showing nuclear localization. Panel e shows the no primary antibody control. The images were captured at 60X magnification.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of Caspase 9 showing staining in the cytoplasm of paraffin-embedded human heart tissue (right) compared to a negative control without primary antibody (left). To expose target proteins, antigen retrieval was performed using 10mM sodium citrate (pH 6.0), microwaved for 8-15 min. Following antigen retrieval, tissues were blocked in 3% H2O2-methanol for 15 min at room temperature, washed with ddH2O and PBS, and then probed with a Caspase 9 Rabbit Polyclonal Antibody (Product # PA5-16358) diluted in 3% BSA-PBS at a dilution of 1:20 for 1 hour at 37°C in a humidified chamber. Tissues were washed extensively in PBST and detection was performed using an HRP-conjugated secondary antibody followed by colorimetric detection using a DAB kit. Tissues were counterstained with hematoxylin and dehydrated with ethanol and xylene to prep for mounting.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunoprecipitation of Caspase 9 using Caspase 9 Polyclonal Antibody (Product # PA5-16358) on denatured Human Jurkat Cells.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Figure 7 Western blot analysis: The cell signaling protein expressions of ( a ) BT-474 and ( b ) MDA-MB-231 cells using PBS, PTX alone, TTZ alone, and PTXNR-TTX treatments. The relative intensity of XIAP, actin, cleaved caspase-9, caspase 3, cleaved caspase 3, and cytochrome C is analyzed after 48 h of treatment in ( c ) BT-474 and ( d ) MDA-MB-231 cells using ImageJ/ Fiji. For each protein expression the experiment was replicated at least n = 2-4 times and the average data is presented as mean +- standard deviation. The relative protein expressions are normalized to the untreated control cells and to the housekeeping GAPDH protein expression (hypothesized expression mean, u = 1). The p values for overexpression or downregulation of cytochrome-C, cleaved caspase-3, and XIAP in BT-474 are 0.039, 0.003, and 0.000049, respectively. The p value for the overexpression of cytochrome-C in MDA-MB-231 is 0.047. ***Represents p < 0.05 (for detailed p value calculations, please see SI Table 6 ).