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Western blot
ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
- References [2]
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- Validations
- Western blot [1]
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- Product number
- AF8301 - Provider product page
- Provider
- R&D Systems
- Product name
- Human Caspase-9 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified. Detects human Caspase-9 precursor and the pro + large subunit (LSU) that migrates as a 37 kDa band on SDS-PAGE generated by cleavage at Asp330. Does not detect the pro + LSU that migrates as a 34 kDa band on SDS-PAGE generated by cleavage at Asp315. Therefore, the major epitope detected occurs between Asp315 and Asp330.
- Reactivity
- Human
- Host
- Goat
- Conjugate
- Unconjugated
- Antigen sequence
P55211- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references Ellipticine derivative NSC 338258 represents a potential new antineoplastic agent for the treatment of multiple myeloma.
The B30.2 domain of pyrin, the familial Mediterranean fever protein, interacts directly with caspase-1 to modulate IL-1beta production.
Tian E, Landowski TH, Stephens OW, Yaccoby S, Barlogie B, Shaughnessy JD Jr
Molecular cancer therapeutics 2008 Mar;7(3):500-9
Molecular cancer therapeutics 2008 Mar;7(3):500-9
The B30.2 domain of pyrin, the familial Mediterranean fever protein, interacts directly with caspase-1 to modulate IL-1beta production.
Chae JJ, Wood G, Masters SL, Richard K, Park G, Smith BJ, Kastner DL
Proceedings of the National Academy of Sciences of the United States of America 2006 Jun 27;103(26):9982-7
Proceedings of the National Academy of Sciences of the United States of America 2006 Jun 27;103(26):9982-7
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Supportive validation
- Submitted by
- R&D Systems (provider)
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- Experimental details
- Detection of Human Caspase-9 precursor and pro + large subunit (LSU) by Western Blot. Western blot shows lysates of Jurkat human acute T cell leukemia cell line untreated (-) or treated (+) with 1 μg/mL staurosporine (STS) for for the indicated times. PVDF membrane was probed with 1 µg/mL of Human Caspase-9 Polyclonal Antibody (Catalog # AF8301), followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). Specific bands were detected for Caspase-9 precursor and pro + LSU at approximately 46 and 37 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 6.
