16016-1-AP

antibody from Invitrogen Antibodies

Targeting: ERP44 KIAA0573, PDIA10, TXNDC4

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunoprecipitation (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

16016-1-AP

Provider

Invitrogen Antibodies

Product name

TXNDC4 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Other

Description

Immunogen sequence: WCRFSQMLH PIFEEASDVI KEEFPNENQV VFARVDCDQH SDIAQRYRIS KYPTLKLFRN GMMMKREYRG QRSVKALADY IRQQKSDPIQ EIRDLAEITT LDRSKRNIIG YFEQKDSDNY RVFERVANIL HDDCAFLSAF GDVSKPERYS GDNIIYKPPG HSAPDMVYLG AMTNFDVTYN WIQDKCVPLV REITFENGEE LTEEGLPFLI LFHMKEDTES LEIFQNEVAR QLISEKGTIN FLHADCDKFR HPLLHIQKTP ADCPVIAIDS FRHMYVFGDF KDVLIPGKLK QFVFDLHSGK LHREFHHGPD PTDTAPGEQA QDVASSPPES SFQKLAPSEY RYTLLRDRDE L (57-406 aa encoded by BC005374)

Reactivity

Human

Host

Rabbit

Isotype

IgG

Vial size

150 µL

Concentration

0.25 mg/mL

Storage

-20°C

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

K-562 cells were subjected to SDS PAGE followed by western blot with 16016-1-AP (TXNDC4 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

HeLa cells were subjected to SDS PAGE followed by western blot with 16016-1-AP (TXNDC4 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

MCF7 cells were subjected to SDS PAGE followed by western blot with 16016-1-AP (TXNDC4 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of Hela cells, using ERP44 antibody 16016-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IGG (red).

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human breast cancer tissue slide using 16016-1-AP (TXNDC4 antibody) at dilution of 1:200 (under 10x lens) heat mediated antigen retrieved with Tris-EDTA buffer (pH 9).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human breast cancer tissue slide using 16016-1-AP (TXNDC4 antibody) at dilution of 1:200 (under 40x lens) heat mediated antigen retrieved with Tris-EDTA buffer (pH 9).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human breast cancer using 16016-1-AP (TXNDC4 antibody) at dilution of 1:100 (under 10x lens).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human breast cancer using 16016-1-AP (TXNDC4 antibody) at dilution of 1:100 (under 40x lens).

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

IP result of anti-TXNDC4 (IP:16016-1-AP, 4ug; Detection:16016-1-AP 1:500) with K-562 cells lysate 4000ug.