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LearnA303-075A
antibody from Invitrogen Antibodies
Targeting: ITSN1
ITSN, MGC134948, MGC134949, SH3D1A, SH3P17
Western blot
ImmunoprecipitationAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- Other assay [1]
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- Product number
- A303-075A - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Intersectin 1 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Other
- Description
- The recommended shelf life for this product is 1 year from date of receipt.
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 1 mg/mL
- Storage
- 4° C
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Detection of human and mouse Intersectin-1 by western blot. Samples: Whole cell lysate (15 µg) from HeLa, HEK293T, Jurkat, TCMK-1, and NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified Rabbit anti-Intersectin-1 antibody (Product # A303-075A; lot A303-075A-2) used for WB at 0.1 µg/mL. Detection: Chemiluminescence with an exposure time of 30 seconds.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Detection of human Intersectin 1 by western blot and immunoprecipitation. Samples: Whole cell lysate (5, 15 and 50 µg for WB; 1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-Intersectin 1 antibody A303-075A used for WB at 0.1 µg/ml (A) and 1 µg/ml (B) and used for IP at 6 µg/mg lysate. Intersectin 1 was also immunoprecipitated by rabbit anti-Intersectin 1 antibody BL11132, which recognizes an upstream epitope. Detection: Chemiluminescence with exposure times of 3 minutes (A) and 10 seconds (B).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Detection of human Intersectin-1 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified Rabbit anti-Intersectin-1 antibody (Product # A303-075A; lot A303-075A-2) used for IP at 6 µg per reaction. Intersectin-1 was also immunoprecipitated by a previous lot of this antibody (lot A303-075A-1). For blotting immunoprecipitated Intersectin-1, Product # A303-075A was used at 1 µg/mL. Detection: Chemiluminescence with an exposure time of 10 seconds.
