PA5-27370

antibody from Invitrogen Antibodies

Targeting: DCN DSPG2, SLRR1B

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-27370

Provider

Invitrogen Antibodies

Product name

Decorin Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Recombinant protein fragment

Description

Recommended positive controls: A549, H1299.

Concentration

1 mg/mL

References

Submitted references

Capture and Detection of Circulating Glioma Cells Using the Recombinant VAR2CSA Malaria Protein.

Bang-Christensen SR, Pedersen RS, Pereira MA, Clausen TM, Løppke C, Sand NT, Ahrens TD, Jørgensen AM, Lim YC, Goksøyr L, Choudhary S, Gustavsson T, Dagil R, Daugaard M, Sander AF, Torp MH, Søgaard M, Theander TG, Østrup O, Lassen U, Hamerlik P, Salanti A, Agerbæk MØ
Cells 2019 Aug 28;8(9)

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Leptospira interrogans causes quantitative and morphological disturbances in adherens junctions and other biological groups of proteins in human endothelial cells.

Sato H, Coburn J
PLoS neglected tropical diseases 2017 Jul;11(7):e0005830

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Placental Sequestration of Plasmodium falciparum Malaria Parasites Is Mediated by the Interaction Between VAR2CSA and Chondroitin Sulfate A on Syndecan-1.

Ayres Pereira M, Mandel Clausen T, Pehrson C, Mao Y, Resende M, Daugaard M, Riis Kristensen A, Spliid C, Mathiesen L, E Knudsen L, Damm P, G Theander T, R Hansson S, A Nielsen M, Salanti A
PLoS pathogens 2016 Aug;12(8):e1005831

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Decorin Polyclonal Antibody detects DCN protein by western blot analysis. A. 30 µg A549 whole cell lysate/extract. B. 30 µg H1299 whole cell lysate/extract.10% SDS-PAGE. Decorin Polyclonal Antibody (Product # PA5-27370) dilution: 1:1,000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis was performed on whole cell extracts (30 µg lysate) of PC-3 (Lane 1), MCF7 (Lane 2) and DU 145 (Lane 3). The blot was probed with Anti-Decorin Polyclonal Antibody (Product # PA5-27370, 1:1000 dilution) and detected by chemiluminescence using Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate (Product # A27036, 0.25 µg/ml, 1:4000 dilution). A 100 kDa band corresponding to Decorin was observed across the cell lines tested.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of Decorin in methanol-fixed A549 cells using a Decorin polyclonal antibody (Product # PA5-27370) at a 1:200 dilution.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical analysis of paraffin-embedded TOV112D xenograft, using Decorin (Product # PA5-27370) antibody at 1:100 dilution. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

NULL

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

NULL

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Fig 1 Effect of Leptospira infection on extracellular matrix proteins in endothelial cells detected by immunofluorescence microscopy. (A) collagen type IV, (B) decorin, and (C) laminin in HMEC-1 and HDLEC are shown in green. The nuclei are stained in blue for all panels. Scale bars represent 50 mum. Quantified signal intensity of the host protein is indicated in the right-hand graphs (mean +/- SD, p -value is indicated below each graph, the independent p -values shown as an asterisk are compared to uninfected cells).