A305-864A-T

antibody from Invitrogen Antibodies

Targeting: WIZ ZNF803

Western blot (Supportive data in Antibodypedia)

Immunoprecipitation (Data presented on provider website)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

A305-864A-T

Provider

Invitrogen Antibodies

Product name

WIZ Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Other

Reactivity

Human, Mouse

Host

Rabbit

Isotype

IgG

Vial size

10 µL

Concentration

1 mg/mL

Storage

4° C

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Detection of human and mouse WIZ by western blot. Samples: Whole cell lysate (50 µg) from HEK293T, MCF-7, HeLa, TCMK-1, and NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified Rabbit anti-WIZ antibody A305-864A-M (lot A305-864A-M-1) used for WB at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG (A120-101P). Chemiluminescence with an exposure time of 75 seconds.

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Detection of human WIZ by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from 293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified Rabbit anti-WIZ antibody (Product # A305-864A-T; lot A305-864A-T-1) used for IP at 40 µL per reaction. WIZ was also immunoprecipitated by rabbit anti-WIZ antibody BL24083. For blotting immunoprecipitated WIZ, Product # A305-864A-T was used at 1:250. Detection: Chemiluminescence with an exposure time time of 75 seconds.