Antibody data
- Antibody Data
- Antigen structure
- References [2]
- Comments [0]
- Validations
- Western blot [2]
- Immunohistochemistry [1]
- Chromatin Immunoprecipitation [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- NBP1-80586 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NBP1-80586, RRID:AB_11011659
- Product name
- Rabbit Polyclonal WIZ Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified. Specificity of human WIZ antibody verified on a Protein Array containing target protein plus 383 other non-specific proteins.
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 0.1 ml
- Storage
- Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Submitted references A WIZ/Cohesin/CTCF Complex Anchors DNA Loops to Define Gene Expression and Cell Identity.
Wiz binds active promoters and CTCF-binding sites and is required for normal behaviour in the mouse.
Justice M, Carico ZM, Stefan HC, Dowen JM
Cell reports 2020 Apr 14;31(2):107503
Cell reports 2020 Apr 14;31(2):107503
Wiz binds active promoters and CTCF-binding sites and is required for normal behaviour in the mouse.
Isbel L, Prokopuk L, Wu H, Daxinger L, Oey H, Spurling A, Lawther AJ, Hale MW, Whitelaw E
eLife 2016 Jul 13;5
eLife 2016 Jul 13;5
No comments: Submit comment
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: WIZ Antibody [NBP1-80586] - Anti-Wiz antibody western blotting. Anti-Wiz antibody staining in adult cerebellum, showing the short (~100-120 KDa) isoforms present in embryo head protein extract, as well as a long (~160 KDa) isoform previously reported to be expressed (Matsumoto et al., 1998).DOI:http://dx.doi.org/10.7554/eLife.15082.005. Image collected and cropped by CiteAb from the following publication (https://elifesciences.org/articles/15082), licensed under a CC-BY licence.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: WIZ Antibody [NBP1-80586] - Anti-Wiz antibody western blotting. Western blotting for Wiz using an anti-Wiz antibody (NBP1-80586) is shown for total protein lysates from E12.5 Wiz+/+ and WizMommeD30/MommeD30 embryo head. Three bands at ~100 kD represent Wiz isoforms, consistent with UCSC gene splice variants, in Wiz+/+ sample and are absent in WizMommeD30/ MommeD30 sample. Western blotting with an anti-gamma-Tubulin antibody is shown as a loading control. Image collected and cropped by CiteAb from the following publication (https://elifesciences.org/articles/15082), licensed under a CC-BY licence.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: WIZ Antibody [NBP1-80586] - Staining of human pancreas shows strong nuclear and slightly weaker nuclear positivity in exocrine as well as endocrine cells.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Chromatin Immunoprecipitation: WIZ Antibody [NBP1-80586] - Wiz binds across the genome and at promoter elements. ChIP-seq was performed for Wiz in the adult male cerebellum, a screenshot is shown of a random Wiz-enriched site (* indicated) with the two ChIP-seq replicates and input, from the ~40,000 significantly enriched peaks. Encode data for CTCF and H3K4me3 is also included. Image collected and cropped by CiteAb from the following publication (https://elifesciences.org/articles/15082), licensed under a CC-BY licence.