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Western blot
ELISAAntibody data
- Antibody Data
- Antigen structure
- References [2]
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- Validations
- Western blot [1]
- Flow cytometry [1]
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- Product number
- NBP1-21059 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NBP1-21059, RRID:AB_1660586
- Product name
- Goat Polyclonal WIZ Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified.
- Reactivity
- Human, Mouse
- Host
- Goat
- Isotype
- IgG
- Vial size
- 0.1 mg
- Concentration
- 0.5 mg/ml
- Storage
- Store at -20C. Avoid freeze-thaw cycles.
Submitted references In vivo localization and identification of SUMOylated proteins in the brain of His6-HA-SUMO1 knock-in mice.
Protein lysine methyltransferase G9a acts on non-histone targets.
Tirard M, Hsiao HH, Nikolov M, Urlaub H, Melchior F, Brose N
Proceedings of the National Academy of Sciences of the United States of America 2012 Dec 18;109(51):21122-7
Proceedings of the National Academy of Sciences of the United States of America 2012 Dec 18;109(51):21122-7
Protein lysine methyltransferase G9a acts on non-histone targets.
Rathert P, Dhayalan A, Murakami M, Zhang X, Tamas R, Jurkowska R, Komatsu Y, Shinkai Y, Cheng X, Jeltsch A
Nature chemical biology 2008 Jun;4(6):344-6
Nature chemical biology 2008 Jun;4(6):344-6
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: WIZ Antibody [NBP1-21059] - Human Cerebellum lysate (A) + Blocking peptide (B) (35 ug protein in RIPA buffer). Antibody at 1 ug/mL. Detected by chemiluminescence
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Flow Cytometry: WIZ Antibody [NBP1-21059] - Paraformaldehyde fixed HeLa cells (blue line), permeabilized with 0.5% Triton. Antibody at 10 ug/mL. Primary incubation 1hr followed by Alexa Fluor 488 secondary antibody at 1 ug/mL. IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.
