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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunoprecipitation [1]
- Immunohistochemistry [2]
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- Product number
- GTX105433 - Provider product page
- Provider
- GeneTex
- Proper citation
- GeneTex Cat#GTX105433, RRID:AB_11173300
- Product name
- MEF2C antibody
- Antibody type
- Polyclonal
- Reactivity
- Human, Mouse
- Host
- Rabbit
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Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Sample (30 ?g of whole cell lysate) A: U87-MG 10% SDS PAGE GTX105433 diluted at 1:10000 The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- MEF2C antibody detects MEF2C protein at cytoplasm and nucleus by immunofluorescent analysis.Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: MEF2C protein stained by MEF2C antibody (GTX105433) diluted at 1:1000.Red: phalloidin, a cytoskeleton marker, diluted at 1:200.Scale bar = 10 £gm.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- MEF2C antibody immunoprecipitates MEF2C protein in IP experiments.IP samples: K562 whole cell extractA. Control with 4 £gg of preimmune Rabbit IgGB. Immunoprecipitation of MEF2C protein by 4 £gg MEF2C antibody (GTX105433)5 % SDS-PAGEThe immunoprecipitated MEF2C protein was detected by MEF2C antibody (GTX105433) diluted at 1:1000.[EasyBlot anti-rabbit IgG (GTX221666-01) was used as a secondary reagent]
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- MEF2C antibody detects MEF2C protein at nucleus on Saos2 xenograft by immunohistochemical analysis. Sample: Paraffin-embedded Saos2 xenograft. MEF2C antibody (GTX105433) dilution: 1:500.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- MEF2C antibody detects MEF2C protein at nucleus on mouse fore brain by immunohistochemical analysis. Sample: Paraffin-embedded mouse fore brain. MEF2C antibody (GTX105433) dilution: 1:500.
