NBP1-89468

antibody from Novus Biologicals

Targeting: MEF2C

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

NBP1-89468

Provider

Novus Biologicals

Proper citation

Novus Cat#NBP1-89468, RRID:AB_11004765

Product name

Rabbit Polyclonal MEF2C Antibody

Antibody type

Polyclonal

Description

Immunogen affinity purified. Specificity of human MEF2C antibody verified on a Protein Array containing target protein plus 383 other non-specific proteins.

Reactivity

Human

Host

Rabbit

Isotype

IgG

Vial size

0.1 ml

Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

References

Submitted references

Mef2c regulates transcription of the extracellular matrix protein cartilage link protein 1 in the developing murine heart.

Lockhart MM, Wirrig EE, Phelps AL, Ghatnekar AV, Barth JL, Norris RA, Wessels A
PloS one 2013;8(2):e57073

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Evolutionary conservation of Nkx2.5 autoregulation in the second heart field.

Clark CD, Zhang B, Lee B, Evans SI, Lassar AB, Lee KH
Developmental biology 2013 Feb 1;374(1):198-209

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Differential expression of cartilage and bone-related proteins in pediatric and adult diseased aortic valves.

Wirrig EE, Hinton RB, Yutzey KE
Journal of molecular and cellular cardiology 2011 Mar;50(3):561-9

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MicroRNA-21 dysregulates the expression of MEF2C in neurons in monkey and human SIV/HIV neurological disease.

Yelamanchili SV, Chaudhuri AD, Chen LN, Xiong H, Fox HS
Cell death & disease 2010;1:e77

Western blot

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: MEF2C Antibody [NBP1-89468] - Analysis in control (vector only transfected HEK293T lysate) and MEF2C over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).

Immunohistochemistry

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: MEF2C Antibody [NBP1-89468] - Staining of human cerebral cortex shows high expression.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: MEF2C Antibody [NBP1-89468] - Staining of human pancreas shows low expression as expected.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: MEF2C Antibody [NBP1-89468] - Staining in human cerebral cortex and pancreas tissues using anti-MEF2C antibody. Corresponding MEF2C RNA-seq data are presented for the same tissues.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: MEF2C Antibody [NBP1-89468] - Staining of human tonsil shows moderate nuclear positivity in germinal center cells.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: MEF2C Antibody [NBP1-89468] - Analysis of human cerebral cortex and liver tissues. Corresponding MEF2C RNA-seg data are presented for the same tissues.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: MEF2C Antibody [NBP1-89468] - Staining of human liver shows no postivity in hepatocytes as expected.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: MEF2C Antibody [NBP1-89468] - Staining of human cerebral cortex shows strong nuclear positivity in neurons.