GTX128961

antibody from GeneTex

Targeting: H2AC18 H2A.2, H2A/q, H2AFO, HIST2H2AA, HIST2H2AA3

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunoprecipitation (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

GTX128961

Provider

GeneTex

Product name

Histone H2AK5ac (acetyl Lys5) antibody

Antibody type

Polyclonal

Reactivity

Human

Host

Rabbit

Western blot

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

Histone H2AK5ac (acetyl Lys5) antibody detects Histone H2AK5ac (acetyl Lys5) protein by Western blot analysis.A. 30 μg HeLa whole cell lysate/extract (0.012% DMSO treatment for 18 hr)B. 30 μg HeLa whole cell lysate/extract (0.4 £gM Trichostatin A treatment for 18 hr)15 % SDS-PAGEHistone H2AK5ac (acetyl Lys5) antibody (GTX128961) dilution: 1:10000

Immunocytochemistry

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

Histone H2AK5ac (acetyl Lys5) antibody detects Histone H2AK5ac (acetyl Lys5) protein at nucleus by immunofluorescent analysis. Samples: HeLa cells mock (left) and treated with 0.4 £gM TSA for 18 hrs (right) were fixed in 4% paraformaldehyde at RT for 15 min.Green: Histone H2AK5ac (acetyl Lys5) protein stained by Histone H2AK5ac (acetyl Lys5) antibody (GTX128961) diluted at 1:500.Blue: Hoechst 33342 staining.Scale bar = 10 £gm.

Immunoprecipitation

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

Histone H2AK5ac (acetyl Lys5) antibody immunoprecipitates Histone H2AK5ac (acetyl Lys5) protein in IP experiments. IP Sample: HeLa whole cell lysate/extract A : 30 ug whole cell lysate/extract of Histone H2AK5ac (acetyl Lys5) protein expressing HeLa cells (0.4uM TSA treatment for 18hr) B : Control with 2.5 £gg of pre-immune rabbit IgG C : Immunoprecipitation of Histone H2AK5ac (acetyl Lys5) protein by 2.5 £gg of Histone H2AK5ac (acetyl Lys5) antibody (GTX128961) 12% SDS-PAGE The immunoprecipitated Histone H2AK5ac (acetyl Lys5) protein was detected by Histone H2AK5ac (acetyl Lys5) antibody (GTX128961) diluted at 1 : 10000. EasyBlot anti-rabbit IgG (HRP) (GTX221666-01) was used as a secondary reagent.

Other assay

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

Dotblot analysis of anti-Histone H2AK5ac (acetyl Lys5) antibody with peptide samples. Varied amount of peptide samples were spotted onto positively charged nylon membrane and blotted with Histone H2AK5ac (acetyl Lys5) antibody (GTX128961) 1:5000 dilution.A: Peptide samples of H2A.2K5 unB: Peptide samples of H2A.2K5acC: Peptide samples of H2BK20 unD: Peptide samples of H2BK20acE: Peptide samples of H2BK5 unF: Peptide samples of H2BK5ac

Validation comment

Dot