NB110-74730

antibody from Novus Biologicals

Targeting: OPN1MW CBBM, CBD, COD5, GCP, OPN1MW1

Western blot (Data presented on provider website)

Immunocytochemistry (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

NB110-74730

Provider

Novus Biologicals

Proper citation

Novus Cat#NB110-74730, RRID:AB_1049390

Product name

Rabbit Polyclonal Opsin 1 (Medium Wave) Antibody

Antibody type

Polyclonal

Description

Unpurified.

Reactivity

Mouse, Rat

Host

Rabbit

Isotype

IgG

Vial size

0.1 ml

Concentration

LYOPH

Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

References

Submitted references

Novel molecular mechanisms for Prph2-associated pattern dystrophy.

Chakraborty D, Strayve DG, Makia MS, Conley SM, Kakahel M, Al-Ubaidi MR, Naash MI
FASEB journal : official publication of the Federation of American Societies for Experimental Biology 2020 Jan;34(1):1211-1230

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Prph2 initiates outer segment morphogenesis but maturation requires Prph2/Rom1 oligomerization.

Conley SM, Stuck MW, Watson JN, Zulliger R, Burnett JL, Naash MI
Human molecular genetics 2019 Feb 1;28(3):459-475

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JNK1 Induces Notch1 Expression to Regulate Genes Governing Photoreceptor Production.

Pan M, Hu H, Wang R, Zhou Y, Zhang L, Wang C, Wang Q
Cells 2019 Aug 24;8(9)

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A Mixture of U.S. Food and Drug Administration-Approved Monoaminergic Drugs Protects the Retina From Light Damage in Diverse Models of Night Blindness.

Leinonen H, Choi EH, Gardella A, Kefalov VJ, Palczewski K
Investigative ophthalmology & visual science 2019 Apr 1;60(5):1442-1453

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A Combination of G Protein-Coupled Receptor Modulators Protects Photoreceptors from Degeneration.

Orban T, Leinonen H, Getter T, Dong Z, Sun W, Gao S, Veenstra A, Heidari-Torkabadi H, Kern TS, Kiser PD, Palczewski K
The Journal of pharmacology and experimental therapeutics 2018 Feb;364(2):207-220

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The K153Del PRPH2 mutation differentially impacts photoreceptor structure and function.

Chakraborty D, Conley SM, Zulliger R, Naash MI
Human molecular genetics 2016 Aug 15;25(16):3500-3514

Immunohistochemistry

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: Opsin 1 (Medium Wave) Antibody [NB110-74730] - IF analysis of Opsin 1 in paraffin embedded and frozen mouse retina tissues. Image courtesy of product review submitted by Linda Vuong.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: Opsin 1 (Medium Wave) Antibody [NB110-74730] - IHC-P on paraffin sections of rat eye. The animal was perfused using Autoperfuser at a pressure of 110 mmHg with 300 ml 4% FA and further post fixed overnight before being processed for paraffin embedding. HIER: Tris-EDTA, pH 9 for 20 min. Blocking: 0.2% LFDM in TBST filtered thru 0.2 um. Detection was done using using HRP polymer following manufacturers inctructions. Primary antibody: dilution 1: 1000, incubated 30 min at RT using Autostainer. Sections were counterstained with Harris Hematoxylin.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry: Opsin 1 (Medium Wave) Antibody [NB110-74730] - Retinal whole mount of BALB/c mouse stained against anti M-opsin secondary (AF647) antibody and peanut agglutinin (Fluorescein). This image was submitted via customer Review.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: Opsin 1 (Medium Wave) Antibody [NB110-74730] - IHC-P on paraffin sections of rat eye.The animal was perfused using Autoperfuser at a pressure of 110 mmHg with 300 ml 4% FA and further post fixed overnight before being processed for paraffin embedding. HIER: Tris-EDTA, pH 9 for 20 min.Blocking: 0.2% LFDM in TBST filtered thru 0.2 um.Detection was done using HRP polymer following manufacturers instructions.Primary antibody: dilution 1: 1000, incubated 30 min at RT using Autostainer.Sections were counterstained with Harris Hematoxylin.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: Opsin 1 (Medium Wave) Antibody [NB110-74730] - IHC-P on paraffin sections of mouse eye. The animal was perfused at a pressure of 130 mmHg with 300 ml 4% FA before being processed for paraffin embedding. HIER: Tris-EDTA, pH 9 for 20 min. Blocking: 0.2% LFDM in TBST filtered thru 0.2 um. Primary antibody: dilution 1: 500, incubated 30 min at RT. Sections were counterstained with Harris Hematoxylin.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: Opsin 1 (Medium Wave) Antibody [NB110-74730] - IHC-P on paraffin sections of mouse eye. The animal was perfused at a pressure of 130 mmHg with 300 ml 4% FA before being processed for paraffin embedding. HIER: Tris-EDTA, pH 9 for 20 min. Blocking: 0.2% LFDM in TBST filtered thru 0.2 um. Primary antibody: dilution 1: 500, incubated 30 min at RT. Sections were counterstained with Harris Hematoxylin.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: Opsin 1 (Medium Wave) Antibody [NB110-74730] - IHC-P on paraffin sections of rat eye. The animal was perfused at a pressure of 130 mmHg with 300 ml 4% FA before being processed for paraffin embedding. HIER: Tris-EDTA, pH 9 for 20 min. Blocking: 0.2% LFDM in TBST filtered thru 0.2 um. Primary antibody: dilution 1: 500, incubated 30 min at RT. Sections were counterstained with Harris Hematoxylin.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: Opsin 1 (Medium Wave) Antibody [NB110-74730] - IHC-P on paraffin sections of rat eye. The animal was perfused at a pressure of 130 mmHg with 300 ml 4% FA before being processed for paraffin embedding. HIER: Tris-EDTA, pH 9 for 20 min. Blocking: 0.2% LFDM in TBST filtered thru 0.2 um. Primary antibody: dilution 1: 500, incubated 30 min at RT. Sections were counterstained with Harris Hematoxylin.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: Opsin 1 (Medium Wave) Antibody [NB110-74730] - IHC-P on paraffin sections of mouse eye. The animal was perfused at a pressure of 130 mmHg with 300 ml 4% FA before being processed for paraffin embedding. HIER: Tris-EDTA, pH 9 for 20 min. Blocking: 0.2% LFDM in TBST filtered thru 0.2 um. Primary antibody: dilution 1: 500, incubated 30 min at RT. Sections were counterstained with Harris Hematoxylin.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: Opsin 1 (Medium Wave) Antibody [NB110-74730] - IHC-P on paraffin sections of rat eye. The animal was perfused at a pressure of 130 mmHg with 300 ml 4% FA before being processed for paraffin embedding. HIER: Tris-EDTA, pH 9 for 20 min. Blocking: 0.2% LFDM in TBST filtered thru 0.2 um. Primary antibody: dilution 1: 500, incubated 30 min at RT. Sections were counterstained with Harris Hematoxylin.