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Western blot
ImmunoprecipitationAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- Immunocytochemistry [1]
- Other assay [1]
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Validation data
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- Product number
- PA5-110082 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- PCMT1 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- Sequence of this protein is as follows: KSGGASHSEL IHNLRKNGII KTDKVFEVML ATDRSHYAKC NPYMDSPQSI GFQATISAPH MHAYALELLF DQLHEGAKAL DVGSGSGILT ACFARMVGCT GKVIGIDHIK ELVDDSVNNV RKDDPTLLSS GRVQLVVGDG RMGYAEEAPY DAIHVGAAAP VVPQALIDQL KPGGRLILPV GPAGGNQMLE QYDKLQDGSI KMKPLMGVIY VPLTDKEKQW SRDEL
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 1.11 mg/mL
- Storage
- -20° C, Avoid Freeze/Thaw Cycles
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of PCMT1 in MCF7, HepG2, 293T, A-431, mouse liver, mouse brain, rat brain, rat heart, rat heart. Samples were incubated in polyclonal PCMT1 antibody (Product # PA5-110082) using a dilution of 1:1000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of PCMT1 in MCF7, HepG2, 293T, A-431, mouse liver, mouse brain, rat brain, rat heart, rat heart. Samples were incubated in polyclonal PCMT1 antibody (Product # PA5-110082) using a dilution of 1:1000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10000.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemistry analysis of PCMT1 in HeLa cells. Samples were incubated in polyclonal PCMT1 antibody (Product # PA5-110082) using a dilution of 1:100, followed by DAPI.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunoprecipitation analysis of PCMT1 in HePG2 cells. Samples were incubated in polyclonal PCMT1 antibody (Product # PA5-110082) using a dilution of 1:1000.
