Explore
Validate
Learn
Western blot
ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
- References [3]
- Comments [0]
- Validations
- Western blot [5]
- Immunocytochemistry [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- GTX111393 - Provider product page
- Provider
- GeneTex
- Proper citation
- GeneTex Cat#GTX111393, RRID:AB_10723101
- Product name
- SQSTM1 / P62 antibody
- Antibody type
- Polyclonal
- Reactivity
- Human, Mouse
- Host
- Rabbit
Submitted references miR‑92b promotes autophagy and suppresses viability and invasion in breast cancer by targeting EZH2.
Phthiocerol dimycocerosates promote access to the cytosol and intracellular burden of Mycobacterium tuberculosis in lymphatic endothelial cells.
A Rab20-Dependent Membrane Trafficking Pathway Controls M. tuberculosis Replication by Regulating Phagosome Spaciousness and Integrity.
Liu F, Sang M, Meng L, Gu L, Liu S, Li J, Geng C
International journal of oncology 2018 Oct;53(4):1505-1515
International journal of oncology 2018 Oct;53(4):1505-1515
Phthiocerol dimycocerosates promote access to the cytosol and intracellular burden of Mycobacterium tuberculosis in lymphatic endothelial cells.
Lerner TR, Queval CJ, Fearns A, Repnik U, Griffiths G, Gutierrez MG
BMC biology 2018 Jan 4;16(1):1
BMC biology 2018 Jan 4;16(1):1
A Rab20-Dependent Membrane Trafficking Pathway Controls M. tuberculosis Replication by Regulating Phagosome Spaciousness and Integrity.
Schnettger L, Rodgers A, Repnik U, Lai RP, Pei G, Verdoes M, Wilkinson RJ, Young DB, Gutierrez MG
Cell host & microbe 2017 May 10;21(5):619-628.e5
Cell host & microbe 2017 May 10;21(5):619-628.e5
No comments: Submit comment
Enhanced validation
Supportive validation
- Submitted by
- GeneTex (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- Non-transfected (¡V) and transfected (+) HepG2 whole cell extracts (30 ?g) were separated by 10% SDS-PAGE, and the membrane was blotted with SQSTM1 antibody (GTX111393) diluted at 1:7000.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- SQSTM1 antibody detects SQSTM1 protein by Western blot analysis.A. 20 µg Huh7 whole cell lysate/extract (untreated) B. 20 µg Huh7 whole cell lysate/extract (3uM-Thapsipargin treatment for 12hr)0 % SDS-PAGESQSTM1 antibody (GTX111393) dilution: 1:1500
- Validation comment
- WB
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Sample (30 ug of whole cell lysate) A: Raji B: K562 C: HL-60 D: NCI-H929 10% SDS PAGE GTX111393 diluted at 1:10000
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Untreated (¡V) and treated (+) HepG2 whole cell extracts (30 £gg) were separated by 10% SDS-PAGE, and the membrane was blotted with SQSTM1 antibody (GTX111393) diluted at 1:10000.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Non-transfected (¡V) and transfected (+) HepG2 whole cell extracts (30 ?g) were separated by 10% SDS-PAGE, and the membrane was blotted with SQSTM1 antibody (GTX111393) diluted at 1:7000.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Confocal immunofluorescence analysis (Olympus FV10i) of paraformaldehyde-fixed HeLa, using SQSTM1(GTX111393) antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with GTX11304 (Red) at 1:2000.
