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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [3]
- Immunocytochemistry [2]
- Immunohistochemistry [1]
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- Product number
- F48010 - Provider product page
- Provider
- NSJ Bioreagents
- Product name
- p62 Antibody
- Antibody type
- Polyclonal
- Antigen
- A portion of amino acids 317-346 from the human protein was used as the immunogen for this p62 antibody.
- Description
- Antigen affinity purified antibody
- Reactivity
- Human
- Host
- Rabbit
- Conjugate
- Unconjugated
- Vial size
- 80 µl, 400 µl
- Storage
- Aliquot the p62 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.
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Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Western blot testing of p62 antibody at 1:500 dilution + HeLa lysate; Predicted band size : 47~62 kDa.
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Western blot analysis of p62 antibody pre-incubated without (Lane 1) and with (2) blocking peptide in MCF-7 lysate
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Western blot analysis of p6 antibody and 293 cell lysate (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the SQSTM1/p62 gene (2).
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Fluorescent image of U251 cells stained with p62 antibody at 1:200. Immunoreactivity is localized to autophagic vacuoles in the cytoplasm.
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Immunofluorescence staining of p62 antibody on Methanol-fixed and PFA fixed HeLa cells. Data courtesy of Dr. Eeva-Liisa Eskelinen, University of Helsinki, Finland.
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- IHC analysis of FFPE human hepatocarcinoma tissue stained with the p62 antibody
