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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
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- Product number
- 647701 - Provider product page
- Provider
- BioLegend
- Proper citation
- BioLegend Cat#647701, RRID:AB_2028561
- Product name
- Purified anti-P62
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein
- Description
- The p62 protein, also called sequestosome 1 (SQSTM1), is a ubiquitin-binding scaffold protein that colocalizes with ubiquitinated protein aggregates in many neurodegenerative diseases and proteinopathies of the liver. The protein is able to polymerize via an N-terminal PB1 domain and can interact with ubiquitinated proteins via the C-terminal UBA domain. Also, p62/SQSTM1 binds directly to LC3 and GABARAP family proteins via a specific sequence motif. The protein is itself degraded by autophagy and may serve to link ubiquitinated proteins to the autophagic machinery to enable their degradation in the lysosome. Recent studies have revealed a novel function for p62 in innate immunity.
- Reactivity
- Human
- Host
- Rabbit
- Conjugate
- Unconjugated
- Vial size
- 50 µl
- Storage
- Upon receipt, store frozen at -20 °C.
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Supportive validation
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- BioLegend (provider)
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- Experimental details
- Hela cell extract was resolved by electrophoresis, transferred to nitrocellulose and probed with polyclonal anti-p62 antibody (Clone Poly6477). Proteins were visualized using donkey anti-rabbit secondary antibody conjugated to HRP and a chemiluminescence system.
- Conjugate
- Unconjugated
