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- Antibody Data
- Antigen structure
- References [22]
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- Validations
- Immunocytochemistry [2]
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- Product number
- BML-PW9860-0100 - Provider product page
- Provider
- Enzo Life Sciences
- Product name
- p62 (human) polyclonal antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- Peptide affinity purified.
- Reactivity
- Human
- Host
- Rabbit
- Vial size
- 100 μl
- Storage
- -20°C
- Handling
- Avoid freeze/thaw cycles. After opening, prepare aliquots and store at -20¡C.
Submitted references Time-Point Dependent Activation of Autophagy and the UPS in SOD1G93A Mice Skeletal Muscle.
Potassium Bisperoxo(1,10-phenanthroline)oxovanadate (bpV(phen)) Induces Apoptosis and Pyroptosis and Disrupts the P62-HDAC6 Protein Interaction to Suppress the Acetylated Microtubule-dependent Degradation of Autophagosomes.
Partial rescue of some features of Huntington Disease in the genetic absence of caspase-6 in YAC128 mice.
Regulated degradation of Chk1 by chaperone-mediated autophagy in response to DNA damage.
Autophagy inhibitor LRPPRC suppresses mitophagy through interaction with mitophagy initiator Parkin.
Hyperactivation of mammalian target of rapamycin complex 1 (mTORC1) promotes breast cancer progression through enhancing glucose starvation-induced autophagy and Akt signaling.
p62/sequestosome 1 regulates aggresome formation of pathogenic ataxin-3 with expanded polyglutamine.
Pale body-like inclusion formation and neurodegeneration following depletion of 26S proteasomes in mouse brain neurones are independent of α-synuclein.
Autophagy deficiency by hepatic FIP200 deletion uncouples steatosis from liver injury in NAFLD.
Pathogenic Parkinson's disease mutations across the functional domains of LRRK2 alter the autophagic/lysosomal response to starvation.
Angiotensin-(1-7) inhibits autophagy in the brain of spontaneously hypertensive rats.
Inhibition of nonsense-mediated RNA decay activates autophagy.
Autophagy is induced by UVA and promotes removal of oxidized phospholipids and protein aggregates in epidermal keratinocytes.
Balance between autophagic pathways preserves retinal homeostasis.
Rho guanine nucleotide exchange factor is an NFL mRNA destabilizing factor that forms cytoplasmic inclusions in amyotrophic lateral sclerosis.
Inhibition of LRRK2 kinase activity stimulates macroautophagy.
Proton-shuttling lichen compound usnic acid affects mitochondrial and lysosomal function in cancer cells.
Rapamycin prevents the mutant huntingtin-suppressed GLT-1 expression in cultured astrocytes.
Analysis of macroautophagy by immunohistochemistry.
The bacterial and cellular determinants controlling the recruitment of mTOR to the Salmonella-containing vacuole.
Temporal orchestration of circadian autophagy rhythm by C/EBPβ.
Neuronal inactivation of peroxisome proliferator-activated receptor γ coactivator 1α (PGC-1α) protects mice from diet-induced obesity and leads to degenerative lesions.
Oliván S, Calvo AC, Gasco S, Muñoz MJ, Zaragoza P, Osta R
PloS one 2015;10(8):e0134830
PloS one 2015;10(8):e0134830
Potassium Bisperoxo(1,10-phenanthroline)oxovanadate (bpV(phen)) Induces Apoptosis and Pyroptosis and Disrupts the P62-HDAC6 Protein Interaction to Suppress the Acetylated Microtubule-dependent Degradation of Autophagosomes.
Chen Q, Yue F, Li W, Zou J, Xu T, Huang C, Zhang Y, Song K, Huang G, Xu G, Huang H, Li J, Liu L
The Journal of biological chemistry 2015 Oct 23;290(43):26051-8
The Journal of biological chemistry 2015 Oct 23;290(43):26051-8
Partial rescue of some features of Huntington Disease in the genetic absence of caspase-6 in YAC128 mice.
Wong BK, Ehrnhoefer DE, Graham RK, Martin DD, Ladha S, Uribe V, Stanek LM, Franciosi S, Qiu X, Deng Y, Kovalik V, Zhang W, Pouladi MA, Shihabuddin LS, Hayden MR
Neurobiology of disease 2015 Apr;76:24-36
Neurobiology of disease 2015 Apr;76:24-36
Regulated degradation of Chk1 by chaperone-mediated autophagy in response to DNA damage.
Park C, Suh Y, Cuervo AM
Nature communications 2015 Apr 16;6:6823
Nature communications 2015 Apr 16;6:6823
Autophagy inhibitor LRPPRC suppresses mitophagy through interaction with mitophagy initiator Parkin.
Zou J, Yue F, Li W, Song K, Jiang X, Yi J, Liu L
PloS one 2014;9(4):e94903
PloS one 2014;9(4):e94903
Hyperactivation of mammalian target of rapamycin complex 1 (mTORC1) promotes breast cancer progression through enhancing glucose starvation-induced autophagy and Akt signaling.
Chen Y, Wei H, Liu F, Guan JL
The Journal of biological chemistry 2014 Jan 10;289(2):1164-73
The Journal of biological chemistry 2014 Jan 10;289(2):1164-73
p62/sequestosome 1 regulates aggresome formation of pathogenic ataxin-3 with expanded polyglutamine.
Zhou L, Wang H, Chen D, Gao F, Ying Z, Wang G
International journal of molecular sciences 2014 Aug 25;15(9):14997-5010
International journal of molecular sciences 2014 Aug 25;15(9):14997-5010
Pale body-like inclusion formation and neurodegeneration following depletion of 26S proteasomes in mouse brain neurones are independent of α-synuclein.
Paine SM, Anderson G, Bedford K, Lawler K, Mayer RJ, Lowe J, Bedford L
PloS one 2013;8(1):e54711
PloS one 2013;8(1):e54711
Autophagy deficiency by hepatic FIP200 deletion uncouples steatosis from liver injury in NAFLD.
Ma D, Molusky MM, Song J, Hu CR, Fang F, Rui C, Mathew AV, Pennathur S, Liu F, Cheng JX, Guan JL, Lin JD
Molecular endocrinology (Baltimore, Md.) 2013 Oct;27(10):1643-54
Molecular endocrinology (Baltimore, Md.) 2013 Oct;27(10):1643-54
Pathogenic Parkinson's disease mutations across the functional domains of LRRK2 alter the autophagic/lysosomal response to starvation.
Manzoni C, Mamais A, Dihanich S, McGoldrick P, Devine MJ, Zerle J, Kara E, Taanman JW, Healy DG, Marti-Masso JF, Schapira AH, Plun-Favreau H, Tooze S, Hardy J, Bandopadhyay R, Lewis PA
Biochemical and biophysical research communications 2013 Nov 29;441(4):862-6
Biochemical and biophysical research communications 2013 Nov 29;441(4):862-6
Angiotensin-(1-7) inhibits autophagy in the brain of spontaneously hypertensive rats.
Jiang T, Gao L, Zhu XC, Yu JT, Shi JQ, Tan MS, Lu J, Tan L, Zhang YD
Pharmacological research 2013 May;71:61-8
Pharmacological research 2013 May;71:61-8
Inhibition of nonsense-mediated RNA decay activates autophagy.
Wengrod J, Martin L, Wang D, Frischmeyer-Guerrerio P, Dietz HC, Gardner LB
Molecular and cellular biology 2013 Jun;33(11):2128-35
Molecular and cellular biology 2013 Jun;33(11):2128-35
Autophagy is induced by UVA and promotes removal of oxidized phospholipids and protein aggregates in epidermal keratinocytes.
Zhao Y, Zhang CF, Rossiter H, Eckhart L, König U, Karner S, Mildner M, Bochkov VN, Tschachler E, Gruber F
The Journal of investigative dermatology 2013 Jun;133(6):1629-37
The Journal of investigative dermatology 2013 Jun;133(6):1629-37
Balance between autophagic pathways preserves retinal homeostasis.
Rodríguez-Muela N, Koga H, García-Ledo L, de la Villa P, de la Rosa EJ, Cuervo AM, Boya P
Aging cell 2013 Jun;12(3):478-88
Aging cell 2013 Jun;12(3):478-88
Rho guanine nucleotide exchange factor is an NFL mRNA destabilizing factor that forms cytoplasmic inclusions in amyotrophic lateral sclerosis.
Droppelmann CA, Keller BA, Campos-Melo D, Volkening K, Strong MJ
Neurobiology of aging 2013 Jan;34(1):248-62
Neurobiology of aging 2013 Jan;34(1):248-62
Inhibition of LRRK2 kinase activity stimulates macroautophagy.
Manzoni C, Mamais A, Dihanich S, Abeti R, Soutar MP, Plun-Favreau H, Giunti P, Tooze SA, Bandopadhyay R, Lewis PA
Biochimica et biophysica acta 2013 Dec;1833(12):2900-10
Biochimica et biophysica acta 2013 Dec;1833(12):2900-10
Proton-shuttling lichen compound usnic acid affects mitochondrial and lysosomal function in cancer cells.
Bessadottir M, Egilsson M, Einarsdottir E, Magnusdottir IH, Ogmundsdottir MH, Omarsdottir S, Ogmundsdottir HM
PloS one 2012;7(12):e51296
PloS one 2012;7(12):e51296
Rapamycin prevents the mutant huntingtin-suppressed GLT-1 expression in cultured astrocytes.
Chen LL, Wu JC, Wang LH, Wang J, Qin ZH, Difiglia M, Lin F
Acta pharmacologica Sinica 2012 Mar;33(3):385-92
Acta pharmacologica Sinica 2012 Mar;33(3):385-92
Analysis of macroautophagy by immunohistochemistry.
Rosenfeldt MT, Nixon C, Liu E, Mah LY, Ryan KM
Autophagy 2012 Jun;8(6):963-9
Autophagy 2012 Jun;8(6):963-9
The bacterial and cellular determinants controlling the recruitment of mTOR to the Salmonella-containing vacuole.
Tattoli I, Philpott DJ, Girardin SE
Biology open 2012 Dec 15;1(12):1215-25
Biology open 2012 Dec 15;1(12):1215-25
Temporal orchestration of circadian autophagy rhythm by C/EBPβ.
Ma D, Panda S, Lin JD
The EMBO journal 2011 Sep 6;30(22):4642-51
The EMBO journal 2011 Sep 6;30(22):4642-51
Neuronal inactivation of peroxisome proliferator-activated receptor γ coactivator 1α (PGC-1α) protects mice from diet-induced obesity and leads to degenerative lesions.
Ma D, Li S, Lucas EK, Cowell RM, Lin JD
The Journal of biological chemistry 2010 Dec 10;285(50):39087-95
The Journal of biological chemistry 2010 Dec 10;285(50):39087-95
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Supportive validation
- Submitted by
- Enzo Life Sciences (provider)
- Main image
- Experimental details
- Western blot analysis of wild type p62, GST-tagged (human, recombinant) bound to: (1) glutathione-agarose and (3) ubiquitin-agarose (2: agarose control). Bound species were analysed by PAGE followed by blotting on to PVDF and probing with Prod. No. BML-PW9860.
- Submitted by
- Enzo Life Sciences (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of p62 immunoreactivity is present in neurons of the hippocampus of an Alzheimer patient. Note the intense reaction in the neurofibrillary tangles (dilution 1:1000). Micrograph courtesy of Professor Fred van Leeuwen, University of Maastricht, The Netherlands.
