Explore
Validate
Learn
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- Immunocytochemistry [2]
- Immunohistochemistry [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- AP32187PU-N - Provider product page
- Provider
- Acris Antibodies GmbH
- Product name
- anti SQSTM1 (C-term)
- Antibody type
- Polyclonal
- Antigen
- KLH conjugated synthetic peptide between 324~353 amino acids from the C-terminal region of Human SQSTM1
- Reactivity
- Human
- Host
- Rabbit
- Vial size
- 0.4 ml
- Concentration
- lot specific
No comments: Submit comment
Supportive validation
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- Western blot analysis of SQSTM1 (arrow) using SQSTM1 Antibody Cat.-No AP32187PU-N. 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the SQSTM1 gene (Lane 2) (Origene Technologies).
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- Western blot analysis of SQSTM1 Antibody Cat.-No AP32187PU-NÂ pre-incubated without (Lane 1) and with (Lane 2) blocking peptide in MCF-7 cell line lysate. SQSTM1 Antibody (arrow) was detected using the purified Pab (1:60/250 dilution).
Supportive validation
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- Confocal immunofluorescent analysis of SQSTM1 Antibody Cat.-No AP32187PU-N with Hela cell followed by Alexa Fluor®488-conjugated Goat anti-Rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- Immunofluorescence staining of Autophagy SQSTM1 Antibody Cat.-No AP32187PU-N on Methanol-fixed and PFA fixed HeLa cells. Data courtesy of Dr. Eeva-Liisa Eskelinen, University of Helsinki,Finland.
Supportive validation
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- Formalin-fixed and paraffin-embedded human cancer tissue reacted with the SQSTM1 Antibody Cat.-No AP32187PU-N, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
