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- Antibody Data
- Antigen structure
- References [12]
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- Western blot [2]
- Immunocytochemistry [2]
- Immunohistochemistry [1]
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- Product number
- ABIN1449619 - Provider product page
- Provider
- antibodies-online
- Product name
- anti-Sequestosome 1 (SQSTM1) (C-Term), (AA 324-353) antibody
- Antibody type
- Polyclonal
- Antigen
- KLH conjugated synthetic peptide between 324~353 amino acids from the C-terminal region of Human SQSTM1
- Description
- Protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS
- Reactivity
- Human
- Host
- Rabbit
- Epitope
- C-Term,AA 324-353
- Vial size
- 0.4 mL
- Concentration
- 0.25 mg/mL
- Storage
- Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
- Handling
- Avoid repeated freezing and thawing.
Submitted references p62/SQSTM1 prominently accumulates in renal proximal tubules in nephropathic cystinosis.
Increased hippocampal accumulation of autophagosomes predicts short-term recognition memory impairment in aged mice.
Induction of an incomplete autophagic response by cancer-preventive geranylgeranoic acid (GGA) in a human hepatoma-derived cell line.
Overexpression of the autophagic beclin-1 protein clears mutant ataxin-3 and alleviates Machado-Joseph disease.
Autophagy negatively regulates keratinocyte inflammatory responses via scaffolding protein p62/SQSTM1.
Autophagy induction with RAD001 enhances chemosensitivity and radiosensitivity through Met inhibition in papillary thyroid cancer.
Epidermal growth factor reduces autophagy in intestinal epithelium and in the rat model of necrotizing enterocolitis.
Invasion and multiplication of Helicobacter pylori in gastric epithelial cells and implications for antibiotic resistance.
Transcription factor GATA4 inhibits doxorubicin-induced autophagy and cardiomyocyte death.
Roles of SIRT1 in the acute and restorative phases following induction of inflammation.
Absence of autophagy results in reactive oxygen species-dependent amplification of RLR signaling.
Impaired protein aggregate handling and clearance underlie the pathogenesis of p97/VCP-associated disease.
Sansanwal P, Sarwal MM
Pediatric nephrology (Berlin, Germany) 2012 Nov;27(11):2137-44
Pediatric nephrology (Berlin, Germany) 2012 Nov;27(11):2137-44
Increased hippocampal accumulation of autophagosomes predicts short-term recognition memory impairment in aged mice.
Soontornniyomkij V, Risbrough VB, Young JW, Soontornniyomkij B, Jeste DV, Achim CL
Age (Dordrecht, Netherlands) 2012 Apr;34(2):305-16
Age (Dordrecht, Netherlands) 2012 Apr;34(2):305-16
Induction of an incomplete autophagic response by cancer-preventive geranylgeranoic acid (GGA) in a human hepatoma-derived cell line.
Okamoto K, Sakimoto Y, Imai K, Senoo H, Shidoji Y
The Biochemical journal 2011 Nov 15;440(1):63-71
The Biochemical journal 2011 Nov 15;440(1):63-71
Overexpression of the autophagic beclin-1 protein clears mutant ataxin-3 and alleviates Machado-Joseph disease.
Nascimento-Ferreira I, Santos-Ferreira T, Sousa-Ferreira L, Auregan G, Onofre I, Alves S, Dufour N, Colomer Gould VF, Koeppen A, Déglon N, Pereira de Almeida L
Brain : a journal of neurology 2011 May;134(Pt 5):1400-15
Brain : a journal of neurology 2011 May;134(Pt 5):1400-15
Autophagy negatively regulates keratinocyte inflammatory responses via scaffolding protein p62/SQSTM1.
Lee HM, Shin DM, Yuk JM, Shi G, Choi DK, Lee SH, Huang SM, Kim JM, Kim CD, Lee JH, Jo EK
Journal of immunology (Baltimore, Md. : 1950) 2011 Jan 15;186(2):1248-58
Journal of immunology (Baltimore, Md. : 1950) 2011 Jan 15;186(2):1248-58
Autophagy induction with RAD001 enhances chemosensitivity and radiosensitivity through Met inhibition in papillary thyroid cancer.
Lin CI, Whang EE, Donner DB, Du J, Lorch J, He F, Jiang X, Price BD, Moore FD Jr, Ruan DT
Molecular cancer research : MCR 2010 Sep;8(9):1217-26
Molecular cancer research : MCR 2010 Sep;8(9):1217-26
Epidermal growth factor reduces autophagy in intestinal epithelium and in the rat model of necrotizing enterocolitis.
Maynard AA, Dvorak K, Khailova L, Dobrenen H, Arganbright KM, Halpern MD, Kurundkar AR, Maheshwari A, Dvorak B
American journal of physiology. Gastrointestinal and liver physiology 2010 Sep;299(3):G614-22
American journal of physiology. Gastrointestinal and liver physiology 2010 Sep;299(3):G614-22
Invasion and multiplication of Helicobacter pylori in gastric epithelial cells and implications for antibiotic resistance.
Chu YT, Wang YH, Wu JJ, Lei HY
Infection and immunity 2010 Oct;78(10):4157-65
Infection and immunity 2010 Oct;78(10):4157-65
Transcription factor GATA4 inhibits doxorubicin-induced autophagy and cardiomyocyte death.
Kobayashi S, Volden P, Timm D, Mao K, Xu X, Liang Q
The Journal of biological chemistry 2010 Jan 1;285(1):793-804
The Journal of biological chemistry 2010 Jan 1;285(1):793-804
Roles of SIRT1 in the acute and restorative phases following induction of inflammation.
Zhang Z, Lowry SF, Guarente L, Haimovich B
The Journal of biological chemistry 2010 Dec 31;285(53):41391-401
The Journal of biological chemistry 2010 Dec 31;285(53):41391-401
Absence of autophagy results in reactive oxygen species-dependent amplification of RLR signaling.
Tal MC, Sasai M, Lee HK, Yordy B, Shadel GS, Iwasaki A
Proceedings of the National Academy of Sciences of the United States of America 2009 Feb 24;106(8):2770-5
Proceedings of the National Academy of Sciences of the United States of America 2009 Feb 24;106(8):2770-5
Impaired protein aggregate handling and clearance underlie the pathogenesis of p97/VCP-associated disease.
Ju JS, Miller SE, Hanson PI, Weihl CC
The Journal of biological chemistry 2008 Oct 31;283(44):30289-99
The Journal of biological chemistry 2008 Oct 31;283(44):30289-99
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Supportive validation
- Submitted by
- antibodies-online (provider)
- Main image
- Experimental details
- Western blot analysis of SQSTM1 Antibody Cat.-No AP32187PU-N pre-incubated without (Lane 1) and with (Lane 2) blocking peptide in MCF-7 cell line lysate. SQSTM1 Antibody (arrow) was detected using the purified Pab (1:60/250 dilution).
- Submitted by
- antibodies-online (provider)
- Main image
- Experimental details
- Western blot analysis of SQSTM1 (arrow) using SQSTM1 Antibody Cat.-No AP32187PU-N. 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the SQSTM1 gene (Lane 2) (Origene Technologies).
Supportive validation
- Submitted by
- antibodies-online (provider)
- Main image
- Experimental details
- Confocal immunofluorescent analysis of SQSTM1 Antibody Cat.-No AP32187PU-N with Hela cell followed by Alexa Fluor®488-conjugated Goat anti-Rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).
- Submitted by
- antibodies-online (provider)
- Main image
- Experimental details
- Immunofluorescence staining of Autophagy SQSTM1 Antibody Cat.-No AP32187PU-N on Methanol-fixed and PFA fixed HeLa cells. Data courtesy of Dr. Eeva-Liisa Eskelinen, University of Helsinki,Finland.
Supportive validation
- Submitted by
- antibodies-online (provider)
- Main image
- Experimental details
- Formalin-fixed and paraffin-embedded human cancer tissue reacted with the SQSTM1 Antibody Cat.-No AP32187PU-N, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
