RQ7944

antibody from NSJ Bioreagents

Targeting: OPLAH 5-Opase, OPLA

Western blot (Supportive data in Antibodypedia)

ELISA (Data presented on provider website)

Immunocytochemistry (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

RQ7944

Provider

NSJ Bioreagents

Product name

OPLAH Antibody / 5-oxo-L-prolinase / 5-OPase

Antibody type

Polyclonal

Description

Antigen affinity purified

Reactivity

Human, Mouse, Rat, Simian

Host

Rabbit

Vial size

100 µg

Concentration

0.5mg/ml if reconstituted with 0.2ml sterile DI water

Storage

After reconstitution, the OPLAH antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Western blot

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

Western blot testing of 1) human HepG2, 2) human T-47D, 3) monkey COS-7, 4) rat liver, 5) rat brain, 6) mouse liver and 7) mouse brain tissue lysate with OPLAH antibody. Predicted molecular weight ~137 kDa.

Immunocytochemistry

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

Immunofluorescent staining of FFPE human PC-3 cells with OPLAH antibody (red) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.

Flow cytometry

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

Flow cytometry testing of human HEL cells with OPLAH antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= OPLAH antibody.