NBP2-54695

antibody from Novus Biologicals

Targeting: H4C2 H4/I, H4FI, HIST1H4B

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

NBP2-54695

Provider

Novus Biologicals

Product name

Rabbit Polyclonal HIST1H4B Antibody

Antibody type

Polyclonal

Description

Immunogen affinity purified. Specificity of human, mouse, rat HIST1H4B antibody verified on a Protein Array containing target protein plus 383 other non-specific proteins.

Reactivity

Human, Mouse, Rat

Host

Rabbit

Isotype

IgG

Vial size

100 ul

Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Western blot

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: HIST1H4B Antibody [NBP2-54695] - Lane 1: NIH-3T3 cell lysate (Mouse embryonic fibroblast cells) Lane 2: NBT-II cell lysate (Rat Wistar bladder tumour cells)

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: HIST1H4B Antibody [NBP2-54695] - Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: Human cell line RT-4 Lane 3: Human cell line U-251 MG

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: HIST1H4B Antibody [NBP2-54695] - Staining of human testis shows moderate to strong nuclear positivity in cells in seminiferous ducts.

Immunohistochemistry

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: HIST1H4B Antibody [NBP2-54695] - Staining of human oral mucosa shows strong nuclear positivity in fraction of squamous epithelial cells.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: HIST1H4B Antibody [NBP2-54695] - Staining of human small intestine shows moderate to strong nuclear positivity in glandular cells.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: HIST1H4B Antibody [NBP2-54695] - Staining of human cervix shows moderate to strong nuclear positivity in squamous epithelial cells.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: HIST1H4B Antibody [NBP2-54695] - Staining of human liver shows very weak nuclear positivity in hepatocytes.