12192-1-AP

antibody from Invitrogen Antibodies

Targeting: SERPINA3 AACT, ACT

Western blot (Supportive data in Antibodypedia)

Immunoprecipitation (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

12192-1-AP

Provider

Invitrogen Antibodies

Product name

alpha 1 Antichymotrypsin Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Other

Description

Immunogen sequence: DKNVIFSPL SISTALAFLS LGAHNTTLTE ILKGLKFNLT ETSEAEIHQS FQHLLRTLNQ SSDELQLSMG NAMFVKEQLS LLDRFTEDAK RLYGSEAFAT DFQDSAAAKK LINDYVKNGT RGKITDLIKD LDSQTMMVLV NYIFFKAKWE MPFDPQDTHQ SRFYLSKKKW VMVPMMSLHH LTIPYFRDEE LSCTVVELKY TGNASALFIL PDQDKMEEVE AMLLPETLKR WRDSLEFREI GELYLPKFSI SRDYNLNDIL LQLGIEEAFT SKADLSGITG ARNLAVSQVV HKAVLDVFEE GTEASAATAV KITLLSALVE TRTIVRFNRP FLMIIVPTDT QNIFFMSKVT NPKQA (70-423 aa encoded by B C010530)

Reactivity

Human

Host

Rabbit

Isotype

IgG

Vial size

150 µL

Concentration

0.47 mg/mL

Storage

-20°C

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

COLO 320 cells were subjected to SDS PAGE followed by western blot with 12192-1-AP (alpha 1 Antichymotrypsin antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Human colon tissue were subjected to SDS PAGE followed by western blot with 12192-1-AP (alpha 1 Antichymotrypsin antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Human blood tissue were subjected to SDS PAGE followed by western blot with 12192-1-AP (alpha 1 Antichymotrypsin antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human liver using 12192-1-AP (alpha 1 Antichymotrypsin antibody) at dilution of 1:50 (under 10x lens).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human liver using 12192-1-AP (alpha 1 Antichymotrypsin antibody) at dilution of 1:50 (under 40x lens).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human spleen using 12192-1-AP (alpha 1 Antichymotrypsin antibody) at dilution of 1:50 (under 10x lens).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human spleen using 12192-1-AP (alpha 1 Antichymotrypsin antibody) at dilution of 1:50 (under 40x lens).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human liver tissue slide using 12192-1-AP (alpha 1 Antichymotrypsin antibody at dilution of 1:200 (under 10x lens).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human liver tissue slide using 12192-1-AP (alpha 1 Antichymotrypsin antibody at dilution of 1:200 (under 40x lens).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human tonsillitis tissue slide using 12192-1-AP ( alpha 1 Antichymotrypsin antibody at dilution of 1:200 (under 10x lens). heat mediated antigen retrieved with Tris-EDTA buffer (pH 9).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human tonsillitis tissue slide using 12192-1-AP ( alpha 1 Antichymotrypsin antibody at dilution of 1:200 (under 40x lens). heat mediated antigen retrieved with Tris-EDTA buffer (pH 9).

Flow cytometry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

1X10^6 HepG2 cells were intracellularly stained with 0.2 µg Anti-Human Alpha Antichymotrypsin (Product # 12192-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1,000 (green), and 0.2 µg Control Antibody. Cells were fixed with 90% MeOH.

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

IP result of anti-alpha 1 Antichymotrypsin (IP:12192-1-AP, 4ug; Detection:12192-1-AP 1:500) with COLO 320 cells lysate 2000ug.