Explore
Validate
Learn
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- Immunohistochemistry [4]
- Flow cytometry [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- 66078-1-IG - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- alpha 1 Antichymotrypsin Monoclonal Antibody (3F2F10)
- Antibody type
- Monoclonal
- Antigen
- Other
- Description
- Immunogen sequence: DKNVIFSPL SISTALAFLS LGAHNTTLTE ILKGLKFNLT ETSEAEIHQS FQHLLRTLNQ SSDELQLSMG NAMFVKEQLS LLDRFTEDAK RLYGSEAFAT DFQDSAAAKK LINDYVKNGT RGKITDLIKD LDSQTMMVLV NYIFFKAKWE MPFDPQDTHQ SRFYLSKKKW VMVPMMSLHH LTIPYFRDEE LSCTVVELKY TGNASALFIL PDQDKMEEVE AMLLPETLKR WRDSLEFREI GELYLPKFSI SRDYNLNDIL LQLGIEEAFT SKADLSGITG ARNLAVSQVV HKAVLDVFEE GTEASAATAV KITLLSALVE TRTIVRFNRP FLMIIVPTDT QNIFFMSKVT NPKQA (70-423 aa encoded by B C010530)
- Reactivity
- Human, Porcine
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- 3F2F10
- Vial size
- 150 µL
- Concentration
- 0.77 mg/mL
- Storage
- -20°C
No comments: Submit comment
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Untreated and PNGase F-treated lysates of human colon tissue (25µg/lane) were subjected to SDS PAGE followed by western blot with 66078-1-Ig (alpha 1 Antichymotrypsin antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. PNGase F was obtained from Atagenix (cat.NO. ata808).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Untreated and PNGase F-treated lysates of human plasma tissue were subjected to SDS PAGE followed by western blot with 66078-1-IG (alpha 1 Antichymotrypsin antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. Loading amount: 0.7 µL crude plasma was lysised for each lane. PNGase F was obtained from Atagenix (cat.NO. ata808).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human liver using 66078-1-Ig (alpha 1 Antichymotrypsin antibody) at dilution of 1:50 (under 10x lens).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human liver using 66078-1-Ig (alpha 1 Antichymotrypsin antibody) at dilution of 1:50 (under 40x lens).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human tonsillitis tissue slide using 66078-1-IG (alpha 1 Antichymotrypsin antibody) at dilution of 1:200 (under 10x lens) heat mediated antigen retrieved with Tris-EDTA buffer (pH 9).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human tonsillitis tissue slide using 66078-1-IG (alpha 1 Antichymotrypsin antibody) at dilution of 1:200 (under 40x lens) heat mediated antigen retrieved with Tris-EDTA buffer (pH 9).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- 1X10^6 HepG2 cells were intracellularly stained with 0.2 µg Anti-Human Alpha Antichymotrypsin (Product # 66078-1-IG, Clone:3F2F10) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1,000 (green), and 0.2 µg Control Antibody. Cells were fixed with 90% MeOH.
