PAB28465

antibody from Abnova Corporation

Targeting: PNP NP, PUNP

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PAB28465

Provider

Abnova Corporation

Product name

NP polyclonal antibody

Antibody type

Polyclonal

Description

Rabbit polyclonal antibody raised against recombinant NP

Antigen sequence

YTYEDYKNTAEWLLSHTKHRPQVAIICGSGLGGLT
DKLTQAQIFDYGEIPNFPRSTVPGHAGRLVFGFLN
GRACVMMQGRFHMYEGYPLWKVTFPVRVFHLLGVD
TLVVTNAAGGLNPKFEVGDIMLIRDHINLPGFSGQ
NPLRGPNDERGAPHR


Isotype

IgG

Storage

Store at 4°C. For long term storage store at -20°C.Aliquot to avoid repeated freezing and thawing.

Western blot

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Western blot analysis of Lane 1: NIH-3T3 cell lysate (Mouse embryonic fibroblast cells), Lane 2: NBT-II cell lysate (Rat Wistar bladder tumour cells), Lane 3: PC12 cell lysate (Pheochromocytoma of rat adrenal medulla) with NP polyclonal antibody.

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Western blot analysis of Lane 1: RT-4, Lane 2: U-251 MG, Lane 3: A-431, Lane 4: Liver, Lane 5: Tonsil with NP polyclonal antibody.

Immunohistochemistry

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Immunohistochemical staining of human kidney with NP polyclonal antibody (Cat # PAB28465) shows strong cytoplasmic and nuclear positivity in cells of tubules at 1:200-1:500 dilution.

Validation comment

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)