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LearnR1055PS
antibody from Acris Antibodies GmbH
Targeting: SERPINA1
A1A, A1AT, AAT, alpha-1-antitrypsin, alpha1AT, PI, PI1
Western blot
ELISAAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Western blot [3]
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- Product number
- R1055PS - Provider product page
- Provider
- Acris Antibodies GmbH
- Product name
- anti Alpha-1-antitrypsin
- Antibody type
- Polyclonal
- Antigen
- Alpha-1-antitrypsin from human plasma
- Reactivity
- Human
- Host
- Goat
- Vial size
- 0.1 mg
- Concentration
- 1.0 mg/ml (by UV absorbance at 280 nm)
Submitted references The amounts of alpha 1 antitrypsin protein are reduced in the vascular wall of the acutely dissected human ascending aorta.
Schachner T, Golderer G, Sarg B, Lindner HH, Bonaros N, Mikuz G, Laufer G, Werner ER
European journal of cardio-thoracic surgery : official journal of the European Association for Cardio-thoracic Surgery 2010 Mar;37(3):684-90
European journal of cardio-thoracic surgery : official journal of the European Association for Cardio-thoracic Surgery 2010 Mar;37(3):684-90
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Supportive validation
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- Western blot of goat anti-Alpha-1-antitrypsin antibody: Lane 1: Alpha-1-antitrypsin under reducing conditions. Lane 2: none. Load: 1 µg per lane. Primary antibody: Alpha-1antitrypsin antibody Cat.-No. R1955 at 1/3000 for overnight at 4°C; Secondary antibody: Dylight 488 conjugated donkey anti goat secondary antibody at 1/10,000 for 45 min at RT; Block overnight at 4°C. Predicted/Observed size: 55kDa for Alpha-1-antitrypsin. Other band(s): none.
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- Western blot of Antitrypsin polyclonal antibody-multiplex fluorescent Western blot: Lane 1: Molecular weight. Lane 2: Alpha-1-antitrypsin Cat.-No. R1055 (red), rabbit anti-transferrin, and mouse-anti-GST were used in a multiplex system to detect target proteins under reducing conditions in albumin depleted human serum with 320 ng of added GST. Load: 35 µg per lane. Primary antibody: Each primary antibody at 1/1000 for overnight at 4°C. Secondary antibody: DyLight549 donkey anti-rabbit IgG (green), DyLight 488 donkey anti-mouse IgG (blue), and DyLight 649 donkey anti-goat IgG (red) secondary antibody at 1:10,000 for 30 min at RT. Block at 30 min RT.
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- Western blot of goat anti-Alpha-1-antitrypsin antibody . Lane 1: molecular weight. Lane 2: Human transferrin (green); Alpha 1 anti trypsin Cat.-No. R1055 (red); and human IgG (blue) in a multiplex fluorescent western blot of human serum. Load: 35 µg per lane. Primary antibody: Each primary antibody was diluted to 1/1000 for overnight at 4°C. Secondary antibody: Rabbit IgG (H&L) antibody DyLight⢠649 conjugated (green), goat IgG (H&L) antibody DyLight⢠549 conjugated (red), mouse IgG (H&L) antibody DyLight⢠488 conjugated (blue) secondary antibody at 1/10,000 for ~1hr at 4°C. Block: IRdye blocking buffer overnight at 4°C.
