DCABH-2840

antibody from Creative Diagnostics

Targeting: SERPINA1 A1A, A1AT, AAT, alpha-1-antitrypsin, alpha1AT, PI, PI1

Western blot (Recommended by provider)

Immunocytochemistry (Recommended by provider)

Immunoprecipitation (Recommended by provider)

Immunohistochemistry (Recommended by provider)

Antibody Data

Product number

DCABH-2840

Provider

Creative Diagnostics

Proper citation

Creative Diagnostics Cat#DCABH-2840, RRID:AB_2476727

Product name

Rabbit Anti-Human SERPINA1 monoclonal antibody, clone KG21-14

Antibody type

Monoclonal

Description

Cumulative damage to lung tissue by Neutrophil Elastase is responsible for the development of pulmonary emphysema, an irreversible lung disease characterized by loss of lung elasticity. a 1-antitrypsin (AAT), a 394 amino acid hepatic acute phase protein, predominantly inhibits Neutrophil Elastase. AAT is highly expressed in liver and in cultured hepatoma cells and, to a lesser extent, in macrophages. AAT is a highly polymorphic glycosylated serum protein with characteristic isoelectric-focusing patterns for most variants. The gene encoding AAT maps to a region of human chromosome 14 that includes a related serine protease inhibitor (serpin) gene which encodes corticosteroid-binding globulin. Oxidation of the methionine 358 residue in the active center of AAT results in a dramatic decrease in inhibitory activity towards elastase. AAT also has a moderate affinity for plasmin and Thrombin. AAT deficiency is associated with a 20-30 fold increased risk of precocious pulmonary emphysema.

Reactivity

Human

Host

Rabbit

Conjugate

Unconjugated

Isotype

IgG

Antibody clone number

KG21-14

Vial size

100 _l

Storage

Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.