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LearnGTX83666
antibody from GeneTex
Targeting: SERPINA1
A1A, A1AT, AAT, alpha-1-antitrypsin, alpha1AT, PI, PI1
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [3]
- Immunoprecipitation [1]
- Immunohistochemistry [5]
- Flow cytometry [1]
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Validation data
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- Product number
- GTX83666 - Provider product page
- Provider
- GeneTex
- Proper citation
- GeneTex Cat#GTX83666, RRID:AB_10726727
- Product name
- alpha 1 Antitrypsin antibody [9A1]
- Antibody type
- Monoclonal
- Reactivity
- Human, Canine, Simian
- Host
- Mouse
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Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Western blot analysis of 35ug of cell extracts from human brain tissue lysates using anti-SERPINA1 antibody.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-SERPINA1 monoclonal antibody.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY SERPINA1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-SERPINA1.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- IP analysis was performed with 500ul of DDK tagged SERPINA1 overexpression lysates and either no antibody control, 2ug of GTX83662 or 2ug of GTX83666. The precipitated protein was detected by DDK tag antibody.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin-embedded Adenocarcinoma of endometrium tissue using anti-SERPINA1mouse monoclonal antibody. (GTX83666, Dilution 1:50)
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin-embedded liver tissue using anti-SERPINA1mouse monoclonal antibody. (GTX83666, Dilution 1:50)
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin-embedded human pancreas tissue using anti-SERPINA1 mouse monoclonal antibody. (GTX83666)
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin-embedded carcinoma of human liver tissue using anti-SERPINA1 mouse monoclonal antibody. (GTX83666)
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin-embedded carcinoma of human thyroid tissue using anti-SERPINA1 mouse monoclonal antibody. (GTX83666)
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- HEK293T cells transfected with either SERPINA1 overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-SERPINA1 antibody (GTX83666), and then analyzed by flow cytometry.
