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LearnV3221
antibody from NSJ Bioreagents
Targeting: SERPINA1
A1A, A1AT, AAT, alpha-1-antitrypsin, alpha1AT, PI, PI1
Western blot
ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [1]
- Other assay [1]
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Validation data
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- Product number
- V3221 - Provider product page
- Provider
- NSJ Bioreagents
- Product name
- Alpha 1 Antitrypsin Antibody / AAT / SERPINA1
- Antibody type
- Monoclonal
- Description
- Purified
- Reactivity
- Human
- Host
- Mouse
- Conjugate
- Unconjugated
- Antibody clone number
- AAT/1378
- Vial size
- 20 µg, 100 µg (with BSA, sodium azide), 100 µg (without BSA, sodium azide), 7 ml (prediluted for IHC)
- Concentration
- 0.2 mg/ml
- Storage
- After reconstitution, the Alpha 1 Antitrypsin antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.
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Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Western blot testing of 1) a partial recombinant protein, 2) human Jurkat and 3) human A549 lysate with Alpha 1 Antitrypsin antibody (clone AAT/1378). Expected molecular weight: ~47 kDa (unmodified), 52 kDa (glycosylated).
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- IHC testing of FFPE human tonsil with Alpha 1 Antitrypsin antibody (clone AAT/1378). Required HIER: steam sections in pH6, 10mM citrate buffer for 10-20 min.
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- SDS-PAGE Analysis of Purified, BSA-Free Alpha 1 Antitrypsin Antibody (clone AAT/1378). Confirmation of Integrity and Purity of the Antibody.
