16382-1-AP
antibody from Invitrogen Antibodies
Targeting: SERPINA1
A1A, A1AT, AAT, alpha-1-antitrypsin, alpha1AT, PI, PI1
Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [4]
- Immunohistochemistry [2]
- Flow cytometry [1]
- Other assay [1]
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Validation data
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- Product number
- 16382-1-AP - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Alpha-1-Antitrypsin Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Other
- Description
- Immunogen sequence: QGDAAQKTD TSHHDQDHPT FNKITPNLAE FAFSLYRQLA HQSNSTNIFF SPVSIATAFA MLSLGTKADT HDEILEGLNF NLTEIPEAQI HEGFQELLRT LNQPDSQLQL TTGNGLFLSE GLKLVDKFLE DVKKLYHSEA FTVNFGDTEE AKKQINDYVE KGTQGKIVDL VKELDRDTVF ALVNYIFFKG KWERPFEVKD TEEEDFHVDQ ATTVKVPMMK RLGMFNIQHC KKLSSWVLLM KYLGNATAIF FLPDEGKLQH LENELTHDII TKFLENEDRR SASLHLPKLS ITGTYDLKSV LGQLGITKVF SNGADLSGVT EEAPLKLSKA VHKAVLTIDE KGTEAAGAMF LEAIPMSIPP EVKFNKPFVF LMIEQNTKSP LFMGKVVNPT QK (28-418 aa encoded by BC015642)
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 150 µL
- Concentration
- 0.48 mg/mL
- Storage
- -20°C
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Human kidney tissue were subjected to SDS PAGE followed by western blot with 16382-1-AP (Alpha-1-Antitrypsin antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Mouse kidney tissue were subjected to SDS PAGE followed by western blot with 16382-1-AP (Alpha-1-Antitrypsin antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Mouse skin tissue were subjected to SDS PAGE followed by western blot with 16382-1-AP (Alpha-1-Antitrypsin antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Mouse lung tissue were subjected to SDS PAGE followed by western blot with 16382-1-AP (Alpha-1-Antitrypsin antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human liver tissue slide using 16382-1-AP ( Alpha-1-Antitrypsin antibody) at dilution of 1:200 (under 10x lens).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human liver tissue slide using 16382-1-AP ( Alpha-1-Antitrypsin antibody) at dilution of 1:200 (under 40x lens).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- 1X10^6 HepG2 cells were stained with 0.2ug Alpha-1-Antitrypsin antibody (16382-1-AP, red) and control antibody (blue). Fixed with 4% PFA blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L) with dilution 1:1500.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- IP result of anti-Alpha-1-Antitrypsin (IP:16382-1-AP, 4ug; Detection:16382-1-AP 1:1000) with mouse kidney tissue lysate 7200ug.