66135-1-IG

antibody from Invitrogen Antibodies

Targeting: SERPINA1 A1A, A1AT, AAT, alpha-1-antitrypsin, alpha1AT, PI, PI1

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunoprecipitation (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

66135-1-IG

Provider

Invitrogen Antibodies

Product name

Alpha-1-Antitrypsin Monoclonal Antibody (1A9G6)

Antibody type

Monoclonal

Antigen

Other

Description

Immunogen sequence: QGDAAQKTD TSHHDQDHPT FNKITPNLAE FAFSLYRQLA HQSNSTNIFF SPVSIATAFA MLSLGTKADT HDEILEGLNF NLTEIPEAQI HEGFQELLRT LNQPDSQLQL TTGNGLFLSE GLKLVDKFLE DVKKLYHSEA FTVNFGDTEE AKKQINDYVE KGTQGKIVDL VKELDRDTVF ALVNYIFFKG KWERPFEVKD TEEEDFHVDQ ATTVKVPMMK RLGMFNIQHC KKLSSWVLLM KYLGNATAIF FLPDEGKLQH LENELTHDII TKFLENEDRR SASLHLPKLS ITGTYDLKSV LGQLGITKVF SNGADLSGVT EEAPLKLSKA VHKAVLTIDE KGTEAAGAMF LEAIPMSIPP EVKFNKPFVF LMIEQNTKSP LFMGKVVNPT QK (28-418 aa encoded by BC015642)

Reactivity

Human, Mouse, Rat, Porcine

Host

Mouse

Isotype

IgG

Antibody clone number

1A9G6

Vial size

150 µL

Concentration

0.85 mg/mL

Storage

-20°C

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Rat liver tissue were subjected to SDS PAGE followed by western blot with 66135-1-Ig (Alpha-1-Antitrypsin Antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

L02 cells were subjected to SDS PAGE followed by western blot with 66135-1-Ig (Alpha-1-Antitrypsin Antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

HepG2 cells were subjected to SDS PAGE followed by western blot with 66135-1-IG ( Alpha-1-Antitrypsin Antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of HepG2 cells using 66135-1-IG (Alpha-1-Antitrypsin antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Mouse IGG (H+L).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of HepG2 cells using 66135-1-Ig (Alpha-1-Antitrypsin antibody) at dilution of 1:25 and Rhodamine-Goat anti-Mouse IgG.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of HepG2 cells using 66135-1-IG (Alpha-1-Antitrypsin antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Mouse IGG (H+L).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of HepG2 cells using 66135-1-Ig (Alpha-1-Antitrypsin antibody) at dilution of 1:25 and Rhodamine-Goat anti-Mouse IgG.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human liver using 66135-1-Ig (Alpha-1-Antitrypsin antibody) at dilution of 1:50 (under 10x lens).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human liver using 66135-1-Ig (Alpha-1-Antitrypsin antibody) at dilution of 1:50 (under 40x lens).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human liver tissue slide using 66135-1-IG ( Alpha-1-Antitrypsin Antibody) at dilution of 1:1000 (under 10x lens).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human liver tissue slide using 66135-1-IG ( Alpha-1-Antitrypsin Antibody) at dilution of 1:1000 (under 40x lens).

Flow cytometry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

1X10^6 HepG2 cells were intracellularly stained with 0.2 µg Anti-Human Alpha 1 Antitrypsin (Product # 66135-1-IG, Clone:1A9G6) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1,000 (green), and 0.2 µg Mouse IgG1 Isotype Control (66360-1-IG, Clone: T1F8D3F10) (black). Cells were fixed with 4% PFA and permeabilized with 0.1% TritonX-100.

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

IP result of anti-Alpha-1-Antitrypsin (IP:66135-1-IG, 5ug; Detection:66135-1-IG 1:1000) with human plasma tissue lysate 4000ug.