V4356

antibody from NSJ Bioreagents

Targeting: SERPINA1 A1A, A1AT, AAT, alpha-1-antitrypsin, alpha1AT, PI, PI1

Immunohistochemistry (Supportive data in Antibodypedia)

Protein array (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

V4356

Provider

NSJ Bioreagents

Product name

Alpha 1 Antitrypsin Antibody / AAT / SERPINA1

Antibody type

Monoclonal

Description

Purified

Reactivity

Human

Host

Mouse

Antibody clone number

AAT/6323

Vial size

20 µg, 100 µg (with BSA, sodium azide), 100 µg (without BSA, sodium azide)

Concentration

0.2 mg/ml, 1 mg/ml

Storage

Aliquot the SERPINA1 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Immunohistochemistry

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

IHC staining of FFPE human ovarian carcinoma tissue with SERPINA1 antibody (clone AAT/6323). Inset: PBS used in place of primary Ab (secondary Ab negative control). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

IHC staining of FFPE human liver in colon with SERPINA1 antibody (AAT/6323). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.

Protein array

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

Analysis of a HuProt(TM) microarray containing more than 19,000 full-length human proteins using SERPINA1 antibody (AAT/6323). Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProt(TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProt(TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a mAb to its intended target. A mAb is considered to specific to its intended target, if the mAb has an S-score of at least 2.5. For example, if a mAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that mAb to protein X is equal to 29.

Other assay

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

SDS-PAGE analysis of purified, BSA-free SERPINA1 antibody (AAT/6323) as confirmation of integrity and purity.