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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [2]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [2]
- Immunohistochemistry [1]
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- Product number
- PA5-21409 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- DIAPH1 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Description
- Recommended positive controls: A431, H1299, HeLa, HepG2, Molt-4, Raji. Predicted reactivity: Mouse (95%), Rat (94%), Pig (98%). Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 0.69 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Submitted references Wnt-controlled sphingolipids modulate Anthrax Toxin Receptor palmitoylation to regulate oriented mitosis in zebrafish.
Periodic propagating waves coordinate RhoGTPase network dynamics at the leading and trailing edges during cell migration.
Castanon I, Hannich JT, Abrami L, Huber F, Dubois M, Müller M, van der Goot FG, Gonzalez-Gaitan M
Nature communications 2020 Jul 3;11(1):3317
Nature communications 2020 Jul 3;11(1):3317
Periodic propagating waves coordinate RhoGTPase network dynamics at the leading and trailing edges during cell migration.
Bolado-Carrancio A, Rukhlenko OS, Nikonova E, Tsyganov MA, Wheeler A, Garcia-Munoz A, Kolch W, von Kriegsheim A, Kholodenko BN
eLife 2020 Jul 24;9
eLife 2020 Jul 24;9
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using DIAPH1 Polyclonal Antibody (Product # PA5-21409). Sample (30 µg of whole cell lysate). Lane A: H1299. Lane B: Raji. 7.5% SDS PAGE. DIAPH1 Polyclonal Antibody (Product # PA5-21409) diluted at 1:1,000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of Dia 1 in paraformaldehyde-fixed HeLa cells using a Dia 1 polyclonal antibody (Product # PA5-21409) (Green) at a 1:500 dilution. Alpha-tubulin filaments were labeled with Product # PA5-29281 (Red) at a 1:2000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Confocal immunofluorescence analysis (Olympus FV10i) of paraformaldehyde-fixed HeLa, using DIAPH1 antibody (Product # PA5-21409) (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with (Product # MA1-25054) (Red) at 1:2,000.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded human gastric tissue, using DIAPH1 (Product # PA5-21409) antibody at 1:100 dilution. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.
