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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [2]
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- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunohistochemistry [2]
- Other assay [1]
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- Product number
- PA5-27607 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- DIAPH1 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Description
- Recommended positive controls: H1299, Raji.
- Concentration
- 1 mg/mL
Submitted references Formin Activity and mDia1 Contribute to Maintain Axon Initial Segment Composition and Structure.
Extension of the clinical and molecular phenotype of DIAPH1-associated autosomal dominant hearing loss (DFNA1).
Zhang W, Ciorraga M, Mendez P, Retana D, Boumedine-Guignon N, Achón B, Russier M, Debanne D, Garrido JJ
Molecular neurobiology 2021 Dec;58(12):6153-6169
Molecular neurobiology 2021 Dec;58(12):6153-6169
Extension of the clinical and molecular phenotype of DIAPH1-associated autosomal dominant hearing loss (DFNA1).
Neuhaus C, Lang-Roth R, Zimmermann U, Heller R, Eisenberger T, Weikert M, Markus S, Knipper M, Bolz HJ
Clinical genetics 2017 Jun;91(6):892-901
Clinical genetics 2017 Jun;91(6):892-901
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using DIAPH1 Polyclonal Antibody (Product # PA5-27607). Sample (30 µg of whole cell lysate). Lane A: H1299. Lane B: Raji . 5% SDS PAGE. DIAPH1 Polyclonal Antibody (Product # PA5-27607) diluted at 1:1,000.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of DIAPH1 in methanol-fixed HeLa cells using a DIAPH1 polyclonal antibody (Product # PA5-27607) (Green) at a 1:500 dilution. Alpha-tubulin filaments were labeled with Product # PA5-29281 (Red) at a 1:2000.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- DIAPH1 Polyclonal Antibody detects DIAPH1 protein at cytoplasm on human colon carcinoma by immunohistochemical analysis. Sample: Paraffin-embedded colon carcinoma. DIAPH1 Polyclonal Antibody (Product # PA5-27607) dilution: 1:250. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- DIAPH1 Polyclonal Antibody detects DIAPH1 protein at membrane on mouse lung by immunohistochemical analysis. Sample: Paraffin-embedded mouse lung. DIAPH1 Polyclonal Antibody (Product # PA5-27607) dilution: 1:500. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Supplementary Figure 5. ( A-C ) Neuro2a cells transfected with srambled (A), shmDia1-1 (B) or shmDia1-2 (C) interference shRNAs for 3 days. Transfected cells are identified by RFP signal (red) and mDia1 knockout validated antibody (ab129167, Abcam) signal is show in green. ( D ) Quantification of mDia1 signal in transfected (red bars) or non-transfected (black bars) Neuro2a cells (n=50 cells/experimental condition). Data are represented as the mean +- SEM. n.s., not significant, ** p < 0.01***, p < 0.0001, Mann-Whitney test. ( E ) 10 DIV hippocampal neurons nucleofected with scrambled interference RNA (scrsh-RFP) or mDia1 interference RNAs (shmDia1-1, shmDia1-2). Nucleofected neurons were identified based on RFP fluorescence (magenta). Neurons were stained with mDia1 (green) and MAP2 (blue) antibodies. Scale bar = 20 mum. ( F ) mDia1 fluorescence intensity was quantified in the soma of neurons nucleofected with scrsh-RFP, shmDia1-1 or shmDia1-2 plasmids. Inserts in D show magnifications of mDia1 staining in soma. **p < 0.01, ***p < 0.001, One-way analysis of variance, Tukey's multiple comparison test. Data in graphs were acquired from three independent experiments and represented as the mean +- SEM. ( G, H ) Axonal and dendritic growth is not affected by mDia1 interference RNA. Hippocampal neurons were nucleofected before plating with shmDia1-1 interference RNA and kept for 3 DIV (G) to analyze axonal length using the tau1 axonal marker, or 7 DIV to analyze total dendritic le
