Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
- Flow cytometry [1]
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Validation data
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- Product number
- ARG54836 - Provider product page
- Provider
- Arigo
- Product name
- anti-CBS antibody
- Antibody type
- Polyclonal
- Antigen
- KLH-conjugated synthetic peptide around aa. 104-133 (N-terminus) of Human CBS.
- Description
- Purification with Protein A and immunogen peptide.
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 200 µl
- Storage
- For continuous use, store undiluted antibody at 2-8°C for up to 6 months. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. The antibody solution should be gently mixed before use.
- Handling
- The antibody solution should be gently mixed before use.
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Supportive validation
- Submitted by
- Arigo (provider)
- Main image
- Experimental details
- Western blot: 35 µg of mouse kidney tissue and Ramos cell line lysates stained with ARG54836 anti-CBS antibody.
- Submitted by
- Arigo (provider)
- Main image
- Experimental details
- Western blot: 2 µg of 293 cell lysates either 1) nontransfected or 2) transiently transfected with the CBS gene stained with ARG54836 anti-CBS antibody.
Supportive validation
- Submitted by
- Arigo (provider)
- Main image
- Experimental details
- Immunofluorescence: 293 cell stained with ARG54836 anti-CBS antibody (green). Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red).DAPI was used to stain the cell nuclear (blue).
Supportive validation
- Submitted by
- Arigo (provider)
- Main image
- Experimental details
- Immunohistochemistry: formalin fixed and paraffin embedded human hepatocarcinoma stained with ARG54836 anti-CBS antibody.
Supportive validation
- Submitted by
- Arigo (provider)
- Main image
- Experimental details
- Flow Cytometry: Ramos cells stained with ARG54836 anti-CBS antibody (bottom histogram) or without primary antibody control (top histogram), followed by incubation with FITC labelled secondary antibody.