Explore
Validate
LearnH00000875-M01
antibody from Novus Biologicals
Targeting: CBS
HIP4
Western blot ELISA
Immunocytochemistry Immunoprecipitation Immunohistochemistry Blocking/NeutralizingAntibody data
- Antibody Data
- Antigen structure
- References [48]
- Comments [0]
- Validations
- Western blot [3]
- ELISA [1]
- Immunoprecipitation [1]
- Immunohistochemistry [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- H00000875-M01 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#H00000875-M01, RRID:AB_537460
- Product name
- Mouse Monoclonal CBS Antibody
- Antibody type
- Monoclonal
- Description
- IgG purified. CBS - cystathionine-beta-synthase
- Reactivity
- Human, Mouse, Rat, Bacteria, Porcine
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 0.1 mg
- Storage
- Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Submitted references Vitamin B-12 and liver activity and expression of methionine synthase are decreased in fetuses with neural tube defects.
A selective and sensitive method for quantification of endogenous polysulfide production in biological samples.
A pharmacological probe identifies cystathionine β-synthase as a new negative regulator for ferroptosis.
Endogenous Hydrogen Sulfide Enhances Cell Proliferation of Human Gastric Cancer AGS Cells.
Downregulation of Glutathione Biosynthesis Contributes to Oxidative Stress and Liver Dysfunction in Acute Kidney Injury.
Maternal N-acetylcysteine therapy regulates hydrogen sulfide-generating pathway and prevents programmed hypertension in male offspring exposed to prenatal dexamethasone and postnatal high-fat diet.
TLR4 upregulates CBS expression through NF-κB activation in a rat model of irritable bowel syndrome with chronic visceral hypersensitivity.
Impacts of CD44 knockdown in cancer cells on tumor and host metabolic systems revealed by quantitative imaging mass spectrometry.
Decreased expression of cystathionine β-synthase promotes glioma tumorigenesis.
Reactive cysteine persulfides and S-polythiolation regulate oxidative stress and redox signaling.
Reduced methylation of PFKFB3 in cancer cells shunts glucose towards the pentose phosphate pathway.
Hypoxia-inducible factors regulate human and rat cystathionine β-synthase gene expression.
Contribution of cysteine aminotransferase and mercaptopyruvate sulfurtransferase to hydrogen sulfide production in peripheral neurons.
Hydrogen sulfide attenuates opioid dependence by suppression of adenylate cyclase/cAMP pathway.
Downregulation of cystathionine β-synthase/hydrogen sulfide contributes to rotenone-induced microglia polarization toward M1 type.
Actions of hydrogen sulfide and ATP-sensitive potassium channels on colonic hypermotility in a rat model of chronic stress.
Sensitization of sodium channels by cystathionine β-synthetase activation in colon sensory neurons in adult rats with neonatal maternal deprivation.
High-fat diet stimulates hepatic cystathionine β-synthase and cystathionine γ-lyase expression.
Promoted interaction of nuclear factor-κB with demethylated cystathionine-β-synthetase gene contributes to gastric hypersensitivity in diabetic rats.
Hydrogen sulfide protects against cellular senescence via S-sulfhydration of Keap1 and activation of Nrf2.
Oxygen-sensitive mitochondrial accumulation of cystathionine β-synthase mediated by Lon protease.
Electroacupuncture suppresses mechanical allodynia and nuclear factor κ B signaling in streptozotocin-induced diabetic rats.
Hydrogen sulphide-induced relaxation of porcine peripheral bronchioles.
Hydrogen sulfide mitigates cardiac remodeling during myocardial infarction via improvement of angiogenesis.
Hydrogen sulfide and resolution of acute inflammation: A comparative study utilizing a novel fluorescent probe.
Hydrogen sulfide producing enzymes in pregnancy and preeclampsia.
Hydrogen sulfide modulates contractile function in rat jejunum.
Interdependency of cystathione γ-lyase and cystathione β-synthase in hydrogen sulfide-induced blood pressure regulation in rats.
Characterization of hydrogen sulfide and its synthases, cystathionine β-synthase and cystathionine γ-lyase, in human prostatic tissue and cells.
Homocystinuria in Taiwan: an inordinately high prevalence in an Austronesian aboriginal tribe, Tao.
Hydrogen Sulfide in the RVLM and PVN has No Effect on Cardiovascular Regulation.
Placental markers of folate-related metabolism in preeclampsia.
Multiple hemodynamic effects of endogenous hydrogen sulfide on central nervous system in rats.
Carbon monoxide stimulates global protein methylation via its inhibitory action on cystathionine β-synthase.
Hydrogen sulfide mediates the anti-survival effect of sulforaphane on human prostate cancer cells.
Rescue of cystathionine beta-synthase (CBS) mutants with chemical chaperones: purification and characterization of eight CBS mutant enzymes.
A crucial role for hydrogen sulfide in oxygen sensing via modulating large conductance calcium-activated potassium channels.
Hydrogen sulphide synthesis in the rat and mouse gastrointestinal tract.
The hydrogen sulfide signaling system: changes during aging and the benefits of caloric restriction.
Astrocytes produce the antiinflammatory and neuroprotective agent hydrogen sulfide.
Actions of hydrogen sulphide on ion transport across rat distal colon.
Endogenous and exogenous hydrogen sulfide promotes resolution of colitis in rats.
The endogenous hydrogen sulfide producing enzyme cystathionine-beta synthase contributes to visceral hypersensitivity in a rat model of irritable bowel syndrome.
Active cystathionine beta-synthase can be expressed in heme-free systems in the presence of metal-substituted porphyrins or a chemical chaperone.
Cystathionine-beta-synthase gene transfer and 3-deazaadenosine ameliorate inflammatory response in endothelial cells.
Hydrogen sulfide enhances ulcer healing in rats.
Hydrogen sulfide is a novel prosecretory neuromodulator in the Guinea-pig and human colon.
Regulation of mitotic chromosome cohesion by Haspin and Aurora B.
Fofou-Caillierez MB, Guéant-Rodriguez RM, Alberto JM, Chéry C, Josse T, Gérard P, Forges T, Foliguet B, Feillet F, Guéant JL
The American journal of clinical nutrition 2019 Mar 1;109(3):674-683
The American journal of clinical nutrition 2019 Mar 1;109(3):674-683
A selective and sensitive method for quantification of endogenous polysulfide production in biological samples.
Bibli SI, Luck B, Zukunft S, Wittig J, Chen W, Xian M, Papapetropoulos A, Hu J, Fleming I
Redox biology 2018 Sep;18:295-304
Redox biology 2018 Sep;18:295-304
A pharmacological probe identifies cystathionine β-synthase as a new negative regulator for ferroptosis.
Wang L, Cai H, Hu Y, Liu F, Huang S, Zhou Y, Yu J, Xu J, Wu F
Cell death & disease 2018 Sep 26;9(10):1005
Cell death & disease 2018 Sep 26;9(10):1005
Endogenous Hydrogen Sulfide Enhances Cell Proliferation of Human Gastric Cancer AGS Cells.
Sekiguchi F, Sekimoto T, Ogura A, Kawabata A
Biological & pharmaceutical bulletin 2016;39(5):887-90
Biological & pharmaceutical bulletin 2016;39(5):887-90
Downregulation of Glutathione Biosynthesis Contributes to Oxidative Stress and Liver Dysfunction in Acute Kidney Injury.
Shang Y, Siow YL, Isaak CK, O K
Oxidative medicine and cellular longevity 2016;2016:9707292
Oxidative medicine and cellular longevity 2016;2016:9707292
Maternal N-acetylcysteine therapy regulates hydrogen sulfide-generating pathway and prevents programmed hypertension in male offspring exposed to prenatal dexamethasone and postnatal high-fat diet.
Tai IH, Sheen JM, Lin YJ, Yu HR, Tiao MM, Chen CC, Huang LT, Tain YL
Nitric oxide : biology and chemistry 2016 Feb 29;53:6-12
Nitric oxide : biology and chemistry 2016 Feb 29;53:6-12
TLR4 upregulates CBS expression through NF-κB activation in a rat model of irritable bowel syndrome with chronic visceral hypersensitivity.
Yuan B, Tang WH, Lu LJ, Zhou Y, Zhu HY, Zhou YL, Zhang HH, Hu CY, Xu GY
World journal of gastroenterology 2015 Jul 28;21(28):8615-28
World journal of gastroenterology 2015 Jul 28;21(28):8615-28
Impacts of CD44 knockdown in cancer cells on tumor and host metabolic systems revealed by quantitative imaging mass spectrometry.
Ohmura M, Hishiki T, Yamamoto T, Nakanishi T, Kubo A, Tsuchihashi K, Tamada M, Toue S, Kabe Y, Saya H, Suematsu M
Nitric oxide : biology and chemistry 2015 Apr 30;46:102-13
Nitric oxide : biology and chemistry 2015 Apr 30;46:102-13
Decreased expression of cystathionine β-synthase promotes glioma tumorigenesis.
Takano N, Sarfraz Y, Gilkes DM, Chaturvedi P, Xiang L, Suematsu M, Zagzag D, Semenza GL
Molecular cancer research : MCR 2014 Oct;12(10):1398-406
Molecular cancer research : MCR 2014 Oct;12(10):1398-406
Reactive cysteine persulfides and S-polythiolation regulate oxidative stress and redox signaling.
Ida T, Sawa T, Ihara H, Tsuchiya Y, Watanabe Y, Kumagai Y, Suematsu M, Motohashi H, Fujii S, Matsunaga T, Yamamoto M, Ono K, Devarie-Baez NO, Xian M, Fukuto JM, Akaike T
Proceedings of the National Academy of Sciences of the United States of America 2014 May 27;111(21):7606-11
Proceedings of the National Academy of Sciences of the United States of America 2014 May 27;111(21):7606-11
Reduced methylation of PFKFB3 in cancer cells shunts glucose towards the pentose phosphate pathway.
Yamamoto T, Takano N, Ishiwata K, Ohmura M, Nagahata Y, Matsuura T, Kamata A, Sakamoto K, Nakanishi T, Kubo A, Hishiki T, Suematsu M
Nature communications 2014 Mar 17;5:3480
Nature communications 2014 Mar 17;5:3480
Hypoxia-inducible factors regulate human and rat cystathionine β-synthase gene expression.
Takano N, Peng YJ, Kumar GK, Luo W, Hu H, Shimoda LA, Suematsu M, Prabhakar NR, Semenza GL
The Biochemical journal 2014 Mar 1;458(2):203-11
The Biochemical journal 2014 Mar 1;458(2):203-11
Contribution of cysteine aminotransferase and mercaptopyruvate sulfurtransferase to hydrogen sulfide production in peripheral neurons.
Miyamoto R, Otsuguro K, Yamaguchi S, Ito S
Journal of neurochemistry 2014 Jul;130(1):29-40
Journal of neurochemistry 2014 Jul;130(1):29-40
Hydrogen sulfide attenuates opioid dependence by suppression of adenylate cyclase/cAMP pathway.
Yang HY, Wu ZY, Wood M, Whiteman M, Bian JS
Antioxidants & redox signaling 2014 Jan 1;20(1):31-41
Antioxidants & redox signaling 2014 Jan 1;20(1):31-41
Downregulation of cystathionine β-synthase/hydrogen sulfide contributes to rotenone-induced microglia polarization toward M1 type.
Du C, Jin M, Hong Y, Li Q, Wang XH, Xu JM, Wang F, Zhang Y, Jia J, Liu CF, Hu LF
Biochemical and biophysical research communications 2014 Aug 22;451(2):239-45
Biochemical and biophysical research communications 2014 Aug 22;451(2):239-45
Actions of hydrogen sulfide and ATP-sensitive potassium channels on colonic hypermotility in a rat model of chronic stress.
Liu Y, Luo H, Liang C, Xia H, Xu W, Chen J, Chen M
PloS one 2013;8(2):e55853
PloS one 2013;8(2):e55853
Sensitization of sodium channels by cystathionine β-synthetase activation in colon sensory neurons in adult rats with neonatal maternal deprivation.
Hu S, Xu W, Miao X, Gao Y, Zhu L, Zhou Y, Xiao Y, Xu GY
Experimental neurology 2013 Oct;248:275-85
Experimental neurology 2013 Oct;248:275-85
High-fat diet stimulates hepatic cystathionine β-synthase and cystathionine γ-lyase expression.
Hwang SY, Sarna LK, Siow YL, O K
Canadian journal of physiology and pharmacology 2013 Nov;91(11):913-9
Canadian journal of physiology and pharmacology 2013 Nov;91(11):913-9
Promoted interaction of nuclear factor-κB with demethylated cystathionine-β-synthetase gene contributes to gastric hypersensitivity in diabetic rats.
Zhang HH, Hu J, Zhou YL, Hu S, Wang YM, Chen W, Xiao Y, Huang LY, Jiang X, Xu GY
The Journal of neuroscience : the official journal of the Society for Neuroscience 2013 May 22;33(21):9028-38
The Journal of neuroscience : the official journal of the Society for Neuroscience 2013 May 22;33(21):9028-38
Hydrogen sulfide protects against cellular senescence via S-sulfhydration of Keap1 and activation of Nrf2.
Yang G, Zhao K, Ju Y, Mani S, Cao Q, Puukila S, Khaper N, Wu L, Wang R
Antioxidants & redox signaling 2013 May 20;18(15):1906-19
Antioxidants & redox signaling 2013 May 20;18(15):1906-19
Oxygen-sensitive mitochondrial accumulation of cystathionine β-synthase mediated by Lon protease.
Teng H, Wu B, Zhao K, Yang G, Wu L, Wang R
Proceedings of the National Academy of Sciences of the United States of America 2013 Jul 30;110(31):12679-84
Proceedings of the National Academy of Sciences of the United States of America 2013 Jul 30;110(31):12679-84
Electroacupuncture suppresses mechanical allodynia and nuclear factor κ B signaling in streptozotocin-induced diabetic rats.
Shi L, Zhang HH, Xiao Y, Hu J, Xu GY
CNS neuroscience & therapeutics 2013 Feb;19(2):83-90
CNS neuroscience & therapeutics 2013 Feb;19(2):83-90
Hydrogen sulphide-induced relaxation of porcine peripheral bronchioles.
Rashid S, Heer JK, Garle MJ, Alexander SP, Roberts RE
British journal of pharmacology 2013 Apr;168(8):1902-10
British journal of pharmacology 2013 Apr;168(8):1902-10
Hydrogen sulfide mitigates cardiac remodeling during myocardial infarction via improvement of angiogenesis.
Qipshidze N, Metreveli N, Mishra PK, Lominadze D, Tyagi SC
International journal of biological sciences 2012;8(4):430-41
International journal of biological sciences 2012;8(4):430-41
Hydrogen sulfide and resolution of acute inflammation: A comparative study utilizing a novel fluorescent probe.
Dufton N, Natividad J, Verdu EF, Wallace JL
Scientific reports 2012;2:499
Scientific reports 2012;2:499
Hydrogen sulfide producing enzymes in pregnancy and preeclampsia.
Holwerda KM, Bos EM, Rajakumar A, Ris-Stalpers C, van Pampus MG, Timmer A, Erwich JJ, Faas MM, van Goor H, Lely AT
Placenta 2012 Jun;33(6):518-21
Placenta 2012 Jun;33(6):518-21
Hydrogen sulfide modulates contractile function in rat jejunum.
Kasparek MS, Linden DR, Farrugia G, Sarr MG
The Journal of surgical research 2012 Jun 15;175(2):234-42
The Journal of surgical research 2012 Jun 15;175(2):234-42
Interdependency of cystathione γ-lyase and cystathione β-synthase in hydrogen sulfide-induced blood pressure regulation in rats.
Roy A, Khan AH, Islam MT, Prieto MC, Majid DS
American journal of hypertension 2012 Jan;25(1):74-81
American journal of hypertension 2012 Jan;25(1):74-81
Characterization of hydrogen sulfide and its synthases, cystathionine β-synthase and cystathionine γ-lyase, in human prostatic tissue and cells.
Guo H, Gai JW, Wang Y, Jin HF, Du JB, Jin J
Urology 2012 Feb;79(2):483.e1-5
Urology 2012 Feb;79(2):483.e1-5
Homocystinuria in Taiwan: an inordinately high prevalence in an Austronesian aboriginal tribe, Tao.
Lu YH, Huang YH, Cheng LM, Yu HC, Hsu JH, Wu TJ, Lo MY, Lin A, Lin CY, Wu JY, Niu DM
Molecular genetics and metabolism 2012 Apr;105(4):590-5
Molecular genetics and metabolism 2012 Apr;105(4):590-5
Hydrogen Sulfide in the RVLM and PVN has No Effect on Cardiovascular Regulation.
Streeter E, Al-Magableh M, Hart JL, Badoer E
Frontiers in physiology 2011;2:55
Frontiers in physiology 2011;2:55
Placental markers of folate-related metabolism in preeclampsia.
Mislanova C, Martsenyuk O, Huppertz B, Obolenskaya M
Reproduction (Cambridge, England) 2011 Sep;142(3):467-76
Reproduction (Cambridge, England) 2011 Sep;142(3):467-76
Multiple hemodynamic effects of endogenous hydrogen sulfide on central nervous system in rats.
Ren YS, Wu SY, Wang XJ, Yu F, Zhao J, Tang CS, Ouyang JP, Geng B
Chinese medical journal 2011 Nov;124(21):3468-75
Chinese medical journal 2011 Nov;124(21):3468-75
Carbon monoxide stimulates global protein methylation via its inhibitory action on cystathionine β-synthase.
Yamamoto T, Takano N, Ishiwata K, Suematsu M
Journal of clinical biochemistry and nutrition 2011 Jan;48(1):96-100
Journal of clinical biochemistry and nutrition 2011 Jan;48(1):96-100
Hydrogen sulfide mediates the anti-survival effect of sulforaphane on human prostate cancer cells.
Pei Y, Wu B, Cao Q, Wu L, Yang G
Toxicology and applied pharmacology 2011 Dec 15;257(3):420-8
Toxicology and applied pharmacology 2011 Dec 15;257(3):420-8
Rescue of cystathionine beta-synthase (CBS) mutants with chemical chaperones: purification and characterization of eight CBS mutant enzymes.
Majtan T, Liu L, Carpenter JF, Kraus JP
The Journal of biological chemistry 2010 May 21;285(21):15866-73
The Journal of biological chemistry 2010 May 21;285(21):15866-73
A crucial role for hydrogen sulfide in oxygen sensing via modulating large conductance calcium-activated potassium channels.
Li Q, Sun B, Wang X, Jin Z, Zhou Y, Dong L, Jiang LH, Rong W
Antioxidants & redox signaling 2010 May 15;12(10):1179-89
Antioxidants & redox signaling 2010 May 15;12(10):1179-89
Hydrogen sulphide synthesis in the rat and mouse gastrointestinal tract.
Martin GR, McKnight GW, Dicay MS, Coffin CS, Ferraz JG, Wallace JL
Digestive and liver disease : official journal of the Italian Society of Gastroenterology and the Italian Association for the Study of the Liver 2010 Feb;42(2):103-9
Digestive and liver disease : official journal of the Italian Society of Gastroenterology and the Italian Association for the Study of the Liver 2010 Feb;42(2):103-9
The hydrogen sulfide signaling system: changes during aging and the benefits of caloric restriction.
Predmore BL, Alendy MJ, Ahmed KI, Leeuwenburgh C, Julian D
Age (Dordrecht, Netherlands) 2010 Dec;32(4):467-81
Age (Dordrecht, Netherlands) 2010 Dec;32(4):467-81
Astrocytes produce the antiinflammatory and neuroprotective agent hydrogen sulfide.
Lee M, Schwab C, Yu S, McGeer E, McGeer PL
Neurobiology of aging 2009 Oct;30(10):1523-34
Neurobiology of aging 2009 Oct;30(10):1523-34
Actions of hydrogen sulphide on ion transport across rat distal colon.
Hennig B, Diener M
British journal of pharmacology 2009 Nov;158(5):1263-75
British journal of pharmacology 2009 Nov;158(5):1263-75
Endogenous and exogenous hydrogen sulfide promotes resolution of colitis in rats.
Wallace JL, Vong L, McKnight W, Dicay M, Martin GR
Gastroenterology 2009 Aug;137(2):569-78, 578.e1
Gastroenterology 2009 Aug;137(2):569-78, 578.e1
The endogenous hydrogen sulfide producing enzyme cystathionine-beta synthase contributes to visceral hypersensitivity in a rat model of irritable bowel syndrome.
Xu GY, Winston JH, Shenoy M, Zhou S, Chen JD, Pasricha PJ
Molecular pain 2009 Aug 6;5:44
Molecular pain 2009 Aug 6;5:44
Active cystathionine beta-synthase can be expressed in heme-free systems in the presence of metal-substituted porphyrins or a chemical chaperone.
Majtan T, Singh LR, Wang L, Kruger WD, Kraus JP
The Journal of biological chemistry 2008 Dec 12;283(50):34588-95
The Journal of biological chemistry 2008 Dec 12;283(50):34588-95
Cystathionine-beta-synthase gene transfer and 3-deazaadenosine ameliorate inflammatory response in endothelial cells.
Sen U, Tyagi N, Kumar M, Moshal KS, Rodriguez WE, Tyagi SC
American journal of physiology. Cell physiology 2007 Dec;293(6):C1779-87
American journal of physiology. Cell physiology 2007 Dec;293(6):C1779-87
Hydrogen sulfide enhances ulcer healing in rats.
Wallace JL, Dicay M, McKnight W, Martin GR
FASEB journal : official publication of the Federation of American Societies for Experimental Biology 2007 Dec;21(14):4070-6
FASEB journal : official publication of the Federation of American Societies for Experimental Biology 2007 Dec;21(14):4070-6
Hydrogen sulfide is a novel prosecretory neuromodulator in the Guinea-pig and human colon.
Schicho R, Krueger D, Zeller F, Von Weyhern CW, Frieling T, Kimura H, Ishii I, De Giorgio R, Campi B, Schemann M
Gastroenterology 2006 Nov;131(5):1542-52
Gastroenterology 2006 Nov;131(5):1542-52
Regulation of mitotic chromosome cohesion by Haspin and Aurora B.
Dai J, Sullivan BA, Higgins JM
Developmental cell 2006 Nov;11(5):741-50
Developmental cell 2006 Nov;11(5):741-50
No comments: Submit comment
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: CBS Antibody (3E1) [H00000875-M01] - Analysis of CBS over-expressed 293 cell line, cotransfected with CBS Validated Chimera RNAi ( Cat # H00000875-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with CBS monoclonal antibody (M01), clone 3E1 (Cat # H00000875-M01 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: CBS Antibody (3E1) [H00000875-M01] - Analysis of CBS expression in transfected 293T cell line by CBS monoclonal antibody (M01), clone 3E1.Lane 1: CBS transfected lysate(61 KDa).Lane 2: Non-transfected lysate.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: CBS Antibody (3E1) [H00000875-M01] - CBS monoclonal antibody (M01), clone 3E1 Analysis of CBS expression in HeLa.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- ELISA: CBS Antibody (3E1) [H00000875-M01] - Detection limit for recombinant GST tagged CBS is approximately 0.1ng/ml as a capture antibody.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunoprecipitation: CBS Antibody (3E1) [H00000875-M01] - Analysis of CBS transfected lysate using anti-CBS monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with CBS MaxPab rabbit polyclonal antibody.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: CBS Antibody (3E1) [H00000875-M01] - Analysis of monoclonal antibody to CBS on formalin-fixed paraffin-embedded human hepatocellular carcinoma. Antibody concentration 3 ug/ml.
