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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [3]
- ELISA [1]
- Immunoprecipitation [1]
- Immunohistochemistry [2]
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- Product number
- AM31730PU-N - Provider product page
- Provider
- Acris Antibodies GmbH
- Proper citation
- Acris Antibodies GmbH Cat#AM31730PU-N, RRID:AB_11001575
- Product name
- anti CBS
- Antibody type
- Monoclonal
- Antigen
- CBS (NP_000062, 1 a.a. ~ 101 a.a) partial recombinant protein with GST tag.
- Reactivity
- Human, Rat
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- 3E1
- Vial size
- 50 µg
- Concentration
- 0.42 mg/ml
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Supportive validation
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- Western Blot analysis of CBS expression in HeLa cell lysate.
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- Western blot analysis of CBS over-expressed 293 cell line, cotransfected with CBS Validated Chimera RNAi (Lane 2) or non-transfected control (Lane 1). Blot probed with CBS antibody, clone 3E1 . GAPDH ( 36.1 kDa ) used as specificity and loading control.
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- Western Blot analysis of CBS expression in transfected 293T cell line. Lane 1: CBS transfected lysate (61 KDa). Lane 2: Non-transfected lysate.
Supportive validation
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- Detection limit for recombinant GST tagged CBS is approximately 0.1ng/ml as a capture antibody.
Supportive validation
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- Immunoprecipitation of CBS transfected lysate using anti-CBS monoclonal antibody and Protein A Magnetic Bead.
Supportive validation
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- Human Brain, Cerebellum: Formalin-Fixed, Paraffin-Embedded (FFPE)
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- Human Liver: Formalin-Fixed, Paraffin-Embedded (FFPE)
