NB100-2863

antibody from Novus Biologicals

Targeting: USP34 KIAA0570, KIAA0729

Western blot (Supportive data in Antibodypedia)

Immunoprecipitation (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

NB100-2863

Provider

Novus Biologicals

Proper citation

Novus Cat#NB100-2863, RRID:AB_2213351

Product name

Rabbit Polyclonal USP34 Antibody

Antibody type

Polyclonal

Description

Immunogen affinity purified.

Reactivity

Human, Mouse

Host

Rabbit

Isotype

IgG

Vial size

0.1 ml

Concentration

0.2 mg/ml

Storage

Store at 4C. Do not freeze.

References

Submitted references

The ubiquitin specific protease USP34 protects the ubiquitin ligase gp78 from proteasomal degradation.

Wang H, Ju D, Kho DH, Yang H, Li L, Raz A, Sun F, Xie Y
Biochemical and biophysical research communications 2019 Feb 5;509(2):348-353

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The ubiquitin-specific protease USP34 regulates axin stability and Wnt/β-catenin signaling.

Lui TT, Lacroix C, Ahmed SM, Goldenberg SJ, Leach CA, Daulat AM, Angers S
Molecular and cellular biology 2011 May;31(10):2053-65

Western blot

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: USP34 Antibody [NB100-2863] - Whole cell lysate from HeLa (5, 15 and 50 mcg for WB; 1 mg for IP, 20% of IP loaded), 293T (T; 50 mcg) and mouse NIH3T3 (M; 50 mcg) cells. NB100-2863 used for WB at 0.04 mcg/ml (A) and 1 mcg/ml (B) and used for IP at 3 mcg/mg lysate (B).

Immunohistochemistry

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry: USP34 Antibody [NB100-2863] - Sample: FFPE section of human prostate carcinoma. Antibody: Affinity purified rabbit anti- USP34 used at a dilution of 1:200 (1ug/ml). Detection: DAB